Patalinghug Jenny Marie Remonde, Padayao Mary Hannah Rose Abadinas, Angeles Isagani Pablo, Yee Jonie Calisogan
Department of Biology, University of San Carlos, Cebu City 6000, Philippines.
Freshwater Fisheries Center of Cagayan Valley, Isabela State University, Echague, Philippines.
Access Microbiol. 2022 Feb 3;4(2):000305. doi: 10.1099/acmi.0.000305. eCollection 2022.
Plant-based protein is being sought after as a substitute for fish meals (powdered fish) in tilapia feeds. This is to promote sustainable aquaculture, as fish meals contribute to the dwindling marine fish catch. is an edible weed that shows potential to improve the growth and immunity of Nile tilapia. However, most studies only consider the survivability of fish to evaluate the benefit of using plant-based feeds and do not necessarily elucidate whether a pathogen is affected . leaf meals (ASLMs) were used to determine effectiveness against (BIOTECH 10089) injected intraperitoneally into Nile tilapia. Formulated feeds with fish meals substituted with 50 % (ASLM) and 75 % (ASLM) leaves were fed to Nile tilapia challenged with . Then spleen and kidney tissue were collected and analysed 10 days post-injection for total plate count. The fish fed with ASLM appeared healthier than those fed with ASLM and those fed with control feeds. Fish fed with ASLMs had lower counts (=0.03). Phytochemical screening and antimicrobial activity determination for crude methanolic leaf (ASL) and ASLMs were also conducted to enhance the results. The metabolites present in the extracts were carbohydrates, amino acids and proteins, cardiac glycosides, saponins and terpenoids. The ASL and ASLM extracts had antimicrobial activity (MIC=115 mg ml). Overall, the study showed that ASLMs can make tilapia more resilient against infections. Fish meal substitution was best at 50 %. Higher substitution had unwanted effects (more bacterial counts), possibly due to antinutritional factors.
植物性蛋白质正被视作罗非鱼饲料中鱼粉（粉状鱼）的替代品。这是为了促进可持续水产养殖，因为鱼粉导致海洋鱼类捕获量不断减少。[植物名称]是一种可食用杂草，显示出改善尼罗罗非鱼生长和免疫力的潜力。然而，大多数研究仅考虑鱼类的存活率来评估使用植物性饲料的益处，并不一定能阐明病原体是否受到影响。[植物名称]叶粉（ASLMs）被用于确定对腹腔注射到尼罗罗非鱼体内的[病原体名称]（BIOTECH 10089）的有效性。用50%（ASLM）和75%（ASLM）[植物名称]叶替代鱼粉的配方饲料喂给受到[病原体名称]攻击的尼罗罗非鱼。然后在注射后10天收集脾脏和肾脏组织并分析其总平板计数。喂食ASLM的鱼看起来比喂食ASLM和对照饲料的鱼更健康。喂食ASLMs的鱼的[细菌名称]计数更低（P = 0.03）。还对粗甲醇[植物名称]叶（ASL）和ASLMs进行了植物化学筛选和抗菌活性测定，以加强[研究结果]。提取物中存在的代谢物有碳水化合物、氨基酸和蛋白质、强心苷、皂苷和萜类化合物。ASL和ASLM提取物具有抗菌活性（MIC = 115毫克/毫升）。总体而言，该研究表明ASLMs可以使罗非鱼对[病原体名称]感染更具抵抗力。鱼粉替代率最佳为50%。更高的替代率有不良影响（细菌计数更多），可能是由于抗营养因子。
