Ren Weifang, Zeng Zhaocong, Wang Sijian, Zhang Juncheng, Fang Jiahai, Wan Liyun
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, China.
Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Nanchang, China.
Front Genet. 2022 Mar 9;13:821163. doi: 10.3389/fgene.2022.821163. eCollection 2022.
Cystathionine γ-synthase (CGS), methionine γ-lyase (MGL), cystathionine β-lyase (CBL) and cystathionine γ-lyase (CGL) share the Cys_Met_Meta_PP domain and play important roles in plant stress response and development. In this study, we defined the genes containing the Cys_Met_Meta_PP domain (PF01053.20) as like genes (). Twenty-nine genes were identified in the peanut genome, including 12 from cultivated peanut and 17 from wild species. These genes were distributed unevenly at the ends of different chromosomes. Evolution, gene structure, and motif analysis revealed that proteins were composed of five different evolutionary branches. Chromosome distribution pattern and synteny analysis strongly indicated that whole-genome duplication (allopolyploidization) contributed to the expansion of genes. Comparative genomics analysis showed that there were three common collinear gene pairs among peanut, Arabidopsis, grape, and soybean, but no collinear gene pairs between peanut and rice. The prediction results of -acting elements showed that , contained different proportions of plant growth, abiotic stress, plant hormones, and light response elements. Spatial expression profiles revealed that almost all had significantly higher expression in pods and seeds. All s could respond to heat stress, and some of them could be rapidly induced by cold, salt, submergence, heat and drought stress. Furthermore, one polymorphic site in was identified by association analysis which was closely associated with pod length (PL), pod width (PW), hundred pod weight (HPW) and hundred seed weight (HSW). The results of this study provide a foundation for further research on the function of the gene family in peanut.
胱硫醚γ-合酶（CGS）、蛋氨酸γ-裂合酶（MGL）、胱硫醚β-裂合酶（CBL）和胱硫醚γ-裂合酶（CGL）共享Cys_Met_Meta_PP结构域，并在植物胁迫响应和发育中发挥重要作用。在本研究中，我们将含有Cys_Met_Meta_PP结构域（PF01053.20）的基因定义为类基因（）。在花生基因组中鉴定出29个基因，其中12个来自栽培花生，17个来自野生种。这些基因在不同染色体末端分布不均。进化、基因结构和基序分析表明，蛋白质由五个不同的进化分支组成。染色体分布模式和共线性分析强烈表明，全基因组复制（异源多倍体化）促成了基因的扩增。比较基因组学分析表明，花生、拟南芥、葡萄和大豆之间有三对常见的共线性基因对，但花生和水稻之间没有共线性基因对。顺式作用元件的预测结果表明，含有不同比例的植物生长、非生物胁迫、植物激素和光响应元件。空间表达谱显示，几乎所有基因在荚果和种子中都有显著更高的表达。所有基因都能响应热胁迫，其中一些能被冷、盐、淹水、热和干旱胁迫快速诱导。此外，通过关联分析在基因中鉴定出一个多态性位点，该位点与荚果长度（PL）、荚果宽度（PW）、百荚重（HPW）和百粒重（HSW）密切相关。本研究结果为进一步研究花生中该基因家族的功能奠定了基础。
