Zhang Cangyu, Deng Rongrong, Zhang Guangzhi, He Xuegang, Chen Haiwei, Chen Bao, Wan Lin, Kang Xuewen
Department of Orthopaedics, The Second Hospital of Lanzhou University, Lanzhou, China.
Key Laboratory of Osteoarthritis of Gansu Province, Lanzhou, China.
Front Neurol. 2022 Mar 10;13:847444. doi: 10.3389/fneur.2022.847444. eCollection 2022.
A systematic review of the role of stem cell-derived exosomes in repairing spinal cord injury (SCI) and the existing problems in animal experiments to provide a reference for better animal experiments and clinical studies in the future.
Three electronic databases, namely PubMed, Web of Science, and Ovid-Embase were searched. The studies were retrieved from inception to October 2021. Two researchers independently screened the literature, extracted data, and evaluated the methodological quality based on the inclusion criteria.
Thirty-two studies were incorporated into the final analyses. Exosomes derived from stem cells could not only significantly improve the motor function of animals with SCI, but also significantly increase the expression of anti-inflammatory factors IL-4 and IL-10 and anti-apoptotic protein Bcl-2, while significantly lowering the pro-inflammatory factor IL-1β and TNF-α and the expression of the apoptotic protein BAX. However, the mechanism of exosome-mediated SCI repair, as well as the best source and dosage remain unknown. In addition, there are still some issues with the design, implementation, and reporting of animal experiments in the included studies. Therefore, future research should further standardize the implementation and reporting of animal studies and fully explore the best strategies for exosomes to repair SCI so as to promote the translation of preclinical research results to clinical research better and faster.
系统综述干细胞衍生外泌体在修复脊髓损伤（SCI）中的作用以及动物实验中存在的问题，为未来更好地开展动物实验和临床研究提供参考。
检索了三个电子数据库，即PubMed、Web of Science和Ovid-Embase。检索时间从数据库建立至2021年10月。两名研究人员根据纳入标准独立筛选文献、提取数据并评估方法学质量。
最终纳入分析32项研究。干细胞衍生的外泌体不仅能显著改善脊髓损伤动物的运动功能，还能显著增加抗炎因子IL-4和IL-10以及抗凋亡蛋白Bcl-2的表达，同时显著降低促炎因子IL-1β和TNF-α以及凋亡蛋白BAX的表达。然而，外泌体介导脊髓损伤修复的机制以及最佳来源和剂量仍不清楚。此外，纳入研究中的动物实验在设计、实施和报告方面仍存在一些问题。因此，未来的研究应进一步规范动物研究的实施和报告，并充分探索外泌体修复脊髓损伤的最佳策略，以便更好、更快地促进临床前研究结果向临床研究的转化。
