Shang Zhizhong, Wang Ruirui, Li Dongliang, Chen Jinlei, Zhang Baolin, Wang Mingchuan, Wang Xin, Wanyan Pingping
The First Clinical Medical School of Lanzhou University, Lanzhou, China.
Chengren Institute of Traditional Chinese Medicine, Lanzhou, China.
Front Pharmacol. 2022 Mar 14;13:819861. doi: 10.3389/fphar.2022.819861. eCollection 2022.
The optimal therapeutic strategies of stem cells for spinal cord injury (SCI) are fully explored in animal studies to promote the translation of preclinical findings to clinical practice, also to provide guidance for future animal experiments and clinical studies. PubMed, Web of Science, Embase, CNKI, Wangfang, VIP, and CBM were searched from inception to September 2021. Screening of search results, data extraction, and references quality evaluation were undertaken independently by two reviewers. A total of 188 studies were included for data analysis. Results of traditional meta-analysis showed that all 15 diverse types of stem cells could significantly improve locomotor function of animals with SCI, and results of further network meta-analysis showed that adipose-derived mesenchymal stem cells had the greatest therapeutic potential for SCI. Moreover, a higher dose (≥1 × 106) of stem cell transplantation had better therapeutic effect, transplantation in the subacute phase (3-14 days, excluding 3 days) was the optimal timing, and intralesional transplantation was the optimal route. However, the evidence of current animal studies is of limited quality, and more high-quality research is needed to further explore the optimal therapeutic strategies of stem cells, while the design and implementation of experiments, as well as measurement and reporting of results for animal studies, need to be further improved and standardized to reduce the risk when the results of animal studies are translated to the clinic. : [website], identifier [registration number].
在动物研究中充分探索了干细胞治疗脊髓损伤（SCI）的最佳治疗策略，以促进临床前研究结果向临床实践的转化，也为未来的动物实验和临床研究提供指导。检索了PubMed、Web of Science、Embase、中国知网、万方、维普和中国生物医学文献数据库，检索时间从建库至2021年9月。由两名评价员独立进行检索结果的筛选、数据提取和参考文献质量评估。共纳入188项研究进行数据分析。传统荟萃分析结果显示，所有15种不同类型的干细胞均可显著改善SCI动物的运动功能，进一步的网状荟萃分析结果显示，脂肪间充质干细胞对SCI具有最大的治疗潜力。此外，较高剂量（≥1×106）的干细胞移植具有更好的治疗效果，亚急性期（3 - 14天，不包括3天）移植是最佳时机，病灶内移植是最佳途径。然而，目前动物研究的证据质量有限，需要更多高质量的研究来进一步探索干细胞的最佳治疗策略，同时动物研究的实验设计与实施以及结果的测量与报告都需要进一步改进和规范，以降低动物研究结果转化至临床时的风险。：[网站]，标识符[注册号]
