Orfinger Alexander B, Morse John C, Hix Raymond L
Center for Water Resources Florida A&M University Tallahassee Florida USA.
Department of Entomology and Nematology University of Florida Gainesville Florida USA.
Ecol Evol. 2022 Mar 24;12(3):e8741. doi: 10.1002/ece3.8741. eCollection 2022 Mar.
Alpha taxonomy of caddisflies (order Trichoptera) is based primarily on male genital morphology. As such, associations of adult females and other life stages typically require conclusive association with the species' identifiable male. The aim of this study was to use molecular methods to associate females and larvae of species represented in the Nearctic. Analysis of mtCOI sequences using distance- and tree-based methods resulted in the association of larvae for 14 species of ( Hamilton, Harris & Lago, 1990, Ross & Yamamoto 1965, Morse 1971, Banks 1905, Ross 1941, Hagen 1861, Smith 1962, Ross 1944, Denning 1950, Milne 1936, Banks 1908, Ross 1941, Yamamoto 1966, and Banks 1900) and females for 2 species ( and Ross & Yamamoto 1965). Searches for, and descriptions of, diagnostic morphological characters for these previously unidentifiable life forms are now possible. The identity of the larva of Banks, 1914 is confirmed and some interesting phylogenetic relationships and a possible cryptic species and potential synonyms are implied in the results. Targets for future immature- and female-male associations are discussed along with a preliminary assessment of morphological differences among larvae.
毛翅目昆虫的科级分类主要基于雄性生殖器形态。因此，成年雌性及其他生命阶段的关联通常需要与该物种可识别的雄性建立明确关联。本研究的目的是使用分子方法将新北区的雌性和幼虫与相应物种进行关联。使用基于距离和树的方法对线粒体细胞色素氧化酶亚基I（mtCOI）序列进行分析，结果实现了14个物种幼虫的关联（汉密尔顿、哈里斯和拉戈，1990年；罗斯和山本，1965年；莫尔斯，1971年；班克斯，1905年；罗斯，1941年；哈根，1861年；史密斯，1962年；罗斯，1944年；丹宁，1950年；米尔恩，1936年；班克斯，1908年；罗斯，1941年；山本，1966年；班克斯，1900年）以及2个物种雌性的关联（罗斯和山本，1965年）。现在有可能对这些以前无法识别的生命形式的诊断形态特征进行搜索和描述。1914年班克斯所描述物种幼虫的身份得到确认，结果中还暗示了一些有趣的系统发育关系以及一个可能的隐存种和潜在的同物异名。文中讨论了未来未成熟个体与雌性 - 雄性关联的目标，并对幼虫之间的形态差异进行了初步评估。
