Awwad Mohammad Hasan, Nada Ossama, Hamdi Momen Mahmoud, El-Shazly Amany Abd El-Fattah, Elwan Sheriff
Ophthalmology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Clin Ophthalmol. 2022 Mar 23;16:893-904. doi: 10.2147/OPTH.S356436. eCollection 2022.
To investigate the correlation between the photopic negative response (PhNR) of the light-adapted flash electroretinography (ERG) and measurements of standard automated perimetry (SAP) and optical coherence tomography (OCT) in assessment of retinal ganglion cells' (RGCs) affection in glaucoma.
A cross-sectional study included 40 eyes of glaucoma patients and 40 eyes of age- and gender-matched normal subjects. Participants underwent a complete ophthalmologic assessment, SAP, OCT, and light-adapted flash ERG using the extended PhNR protocol of the International Society for Clinical Electrophysiology of Vision (ISCEV). Glaucomatous eyes were divided into 3 subgroups: mild (n = 15), moderate (n = 11) and severe glaucoma (n = 14) according to the mean deviation (MD) of SAP. Measurements of SAP, OCT and ERG parameters were analyzed, and correlations between PhNR measurements and other study measurements were evaluated.
PhNR amplitudes and PhNR/b-wave ratios were significantly reduced in glaucoma cases compared to healthy controls, and they showed a significant and progressive decline across the three glaucoma subgroups (P < 0.05). An exception to this is PT (b-wave peak to PhNR trough) PhNR amplitude where its reduction was statistically non-significant when comparing between controls and mild glaucoma cases (P = 0.178), and between moderate and severe glaucoma cases (P = 0.714). PhNR amplitudes and PhNR/b-wave ratios correlated significantly with SAP and OCT parameters (P < 0.05).
PhNR correlates well with SAP and OCT parameters in glaucoma assessment. PhNR could be a valuable supplementary tool for objective assessment of the RGCs' function in glaucoma.
探讨明适应闪光视网膜电图（ERG）的视锥负反应（PhNR）与标准自动视野计（SAP）测量值及光学相干断层扫描（OCT）在评估青光眼视网膜神经节细胞（RGCs）损伤中的相关性。
一项横断面研究纳入了40例青光眼患者的40只眼以及年龄和性别匹配的40例正常受试者的40只眼。参与者接受了全面的眼科评估、SAP、OCT以及使用国际临床视觉电生理学会（ISCEV）扩展PhNR方案的明适应闪光ERG检查。根据SAP的平均偏差（MD），青光眼患眼被分为3个亚组：轻度（n = 15）、中度（n = 11）和重度青光眼（n = 14）。分析了SAP、OCT和ERG参数的测量值，并评估了PhNR测量值与其他研究测量值之间的相关性。
与健康对照组相比，青光眼病例的PhNR振幅和PhNR/b波比值显著降低，并且在三个青光眼亚组中呈显著且逐渐下降趋势（P < 0.05）。例外的是PT（b波峰到PhNR谷）PhNR振幅，在比较对照组与轻度青光眼病例时其降低无统计学意义（P = 0.178），在比较中度与重度青光眼病例时也无统计学意义（P = 0.714）。PhNR振幅和PhNR/b波比值与SAP和OCT参数显著相关（P < 0.05）。
在青光眼评估中，PhNR与SAP和OCT参数具有良好的相关性。PhNR可能是客观评估青光眼RGCs功能的有价值的辅助工具。
