Di Natale Concetta, De Gregorio Vincenza, Lagreca Elena, Mauro Francesca, Corrado Brunella, Vecchione Raffaele, Netti Paolo Antonio
Interdisciplinary Research Centre on Biomaterials, University of Naples Federico II, Naples, Italy.
Istituto Italiano di Tecnologia, Naples, Italy.
Front Bioeng Biotechnol. 2022 Mar 9;10:851893. doi: 10.3389/fbioe.2022.851893. eCollection 2022.
Bacterial cellulose (BC) is a highly pure form of cellulose produced by bacteria, which possesses numerous advantages such as good mechanical properties, high chemical flexibility, and the ability to assemble in nanostructures. Thanks to these features, it achieved a key role in the biomedical field and in drug delivery applications. BC showed its ability to modulate the release of several drugs and biomolecules to the skin, thus improving their clinical outcomes. This work displays the loading of a 3D BC nanonetwork with an innovative drug delivery nanoemulsion system. BC was optimized by static culture of SCOBY (symbiotic colony of bacteria and yeast) and characterized by morphological and ultrastructural analyses, which indicate a cellulose fiber diameter range of 30-50 nm. BC layers were then incubated at different time points with a nanocarrier based on a secondary nanoemulsion (SNE) previously loaded with a well-known antioxidant and anti-inflammatory agent, namely, coenzyme-Q10 (Co-Q10). Incubation of Co-Q10-SNE in the BC nanonetwork and its release were analyzed by fluorescence spectroscopy.
细菌纤维素（BC）是细菌产生的一种高纯度纤维素形式，具有许多优点，如良好的机械性能、高化学柔韧性以及在纳米结构中组装的能力。由于这些特性，它在生物医学领域和药物递送应用中发挥了关键作用。BC显示出能够调节多种药物和生物分子向皮肤的释放，从而改善其临床效果。这项工作展示了一种创新的药物递送纳米乳液系统在3D BC纳米网络中的负载情况。通过SCOBY（细菌和酵母共生菌落）的静态培养对BC进行了优化，并通过形态学和超微结构分析对其进行了表征，结果表明纤维素纤维直径范围为30 - 50纳米。然后，将BC层在不同时间点与基于二次纳米乳液（SNE）的纳米载体孵育，该纳米载体预先负载了一种著名的抗氧化剂和抗炎剂，即辅酶Q10（Co - Q10）。通过荧光光谱法分析了Co - Q10 - SNE在BC纳米网络中的孵育及其释放情况。
