Department of Chemical Science and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy.
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Anal Chem. 2021 Mar 30;93(12):5225-5233. doi: 10.1021/acs.analchem.0c05469. Epub 2021 Mar 19.
The growth of (bio)sensors in analytical chemistry is mainly attributable to the development of affordable, effective, portable, and user-friendly analytical tools. In the field of sensors, paper-based devices are gaining a relevant position for their outstanding features including foldability, ease of use, and instrument-free microfluidics. Herein, a multifarious use of filter paper to detect copper ions in bodily fluids is reported by exploiting this eco-friendly material to (i) synthesize AuNPs without the use of reductants and/or external stimuli, (ii) print the electrodes, (iii) load the reagents for the assay, (iv) filter the gross impurities, and (v) preconcentrate the target analyte. Copper ions were detected down to 3 ppb with a linearity up to 400 ppb in standard solutions. The applicability in biological matrices, namely, sweat and serum, was demonstrated by recovery studies and by analyzing these biofluids with the paper-based platform and the reference method (atomic absorption spectroscopy), demonstrating satisfactory accuracy of the novel eco-designed analytical tool.
生物传感器在分析化学中的发展主要归因于经济实惠、有效、便携和用户友好的分析工具的发展。在传感器领域，纸基器件因其出色的特性，包括可折叠性、易用性和无仪器微流控性，正在获得重要地位。在此，通过利用这种环保材料来（i）在没有还原剂和/或外部刺激的情况下合成 AuNPs，（ii）打印电极，（iii）负载用于检测的试剂，（iv）过滤杂质，和（v）预浓缩目标分析物，报告了滤纸在体液中检测铜离子的多种用途。在标准溶液中，铜离子的检测下限低至 3 ppb，线性范围高达 400 ppb。通过回收率研究并使用基于纸张的平台和参考方法（原子吸收光谱法）分析这些生物流体，证明了其在生物基质（即汗液和血清）中的适用性，表明了新型环保设计分析工具具有令人满意的准确性。
