Li Peichuang, Cai Wanhao, Li Xin, Zhang Hong, Zhao Yuancong, Wang Jin
Heze Branch, Qilu University of Technology (Shandong Academy of Sciences), Biological Engineering Technology Innovation Center of Shandong Province, Heze, China.
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, China.
Front Bioeng Biotechnol. 2022 Mar 9;10:874419. doi: 10.3389/fbioe.2022.874419. eCollection 2022.
In this study, a sulfur-mediated polycarbonate polyurethane (PCU-SS) is developed by mimicking the catalyzing ability of glutathione peroxidase (GPx) on nitric oxide (NO) in the human body. The PCU-SS is endowed with the capability to produce NO based on disulfide bonds, which could strongly improve the biocompatibility of the materials. The characterization results indicate that PCU-SS could not only decrease the adhesion of platelets but also enhance the capability of anti-thrombus. Moreover, it is shown that PCU-SS has a good compatibility with endothelial cells (ECs), while has a marked inhibition capacity of the proliferation of smooth muscle cells (SMCs) and macrophages (MA). Meanwhile, the result of animal implantation experiments further demonstrates the good abilities of PCU-SS on anti-inflammation, anti-thrombus, and anti-hyperplasia. Our results offer a novel strategy for the modification of blood-contacting materials based on disulfide bonds. It is expected that the PCU-SS could shed new light on biocompatibility improvement of cardiovascular stents.
在本研究中，通过模拟人体中谷胱甘肽过氧化物酶（GPx）对一氧化氮（NO）的催化能力，开发了一种硫介导的聚碳酸酯聚氨酯（PCU-SS）。PCU-SS具有基于二硫键产生NO的能力，这可以显著提高材料的生物相容性。表征结果表明，PCU-SS不仅可以降低血小板的粘附，还可以增强抗血栓形成的能力。此外，结果表明PCU-SS与内皮细胞（ECs）具有良好的相容性，同时对平滑肌细胞（SMCs）和巨噬细胞（MA）的增殖具有显著的抑制能力。同时，动物植入实验结果进一步证明了PCU-SS在抗炎、抗血栓和抗增生方面的良好能力。我们的结果为基于二硫键的血液接触材料改性提供了一种新策略。预计PCU-SS可为心血管支架生物相容性的改善提供新的思路。
