[C57BL/6小鼠外周淋巴组织和肝脏中CD11c+B220+NK细胞的分析]

[Analysis of CD11c B220NK cells in peripheral lymphoid tissues and the liver of C57BL/6 mice].

作者信息

Wang Yu, Yuan Xin, Qin Huan, Xu Yun'e, Sun Xin

机构信息

Department of Microbiology, Zunyi Medical University, Zunyi 563000, China. *Corresponding author, E-mail:

Department of Microbiology, Zunyi Medical University, Zunyi 563000, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2022 Feb;38(2):116-121.

PMID:35356879

Abstract

Objective To investigate the distribution of CD11cB220NK cells in peripheral lymphoid tissues and liver and the surface expression of plasmacytoid dendritic cell antigen-1 (PDCA-1) on CD11c B220 NK cells. Methods The spleen, lymph nodes and liver tissues of C57BL/6 mice were collected to prepare single-cell suspensions, and the proportion of CD11cB220NK cells in the tissues and their surface expression of PDCA-1 were detected by multi-color flow cytometry. Results CD11cB220NK cells were distributed widely in the spleen, lymph nodes and liver, with the highest proportion in the spleen (2.82±0.45)%. PDCA-1s were expressed in some of CD11cB220NK cells in the tissues, particularly in the spleen tissues. Conclusion CD11cB220NK cells are important subpopulation of NK cells in murine peripheral lymphoid tissues and liver. The expression of PDCA-1 on CD11cB220NK cells is different in different tissues.

摘要

目的研究CD11c⁺B220⁺NK细胞在小鼠外周淋巴组织及肝脏中的分布情况以及浆细胞样树突状细胞抗原-1（PDCA-1）在CD11c⁺B220⁺NK细胞表面的表达情况。方法采集C57BL/6小鼠的脾脏、淋巴结及肝脏组织，制备单细胞悬液，采用多色流式细胞术检测组织中CD11c⁺B220⁺NK细胞的比例及其表面PDCA-1的表达情况。结果 CD11c⁺B220⁺NK细胞广泛分布于脾脏、淋巴结及肝脏中，脾脏中的比例最高，为（2.82±0.45）%。组织中的部分CD11c⁺B220⁺NK细胞表达PDCA-1，尤其在脾脏组织中。结论 CD11c⁺B220⁺NK细胞是小鼠外周淋巴组织及肝脏中NK细胞的重要亚群。不同组织中CD11c⁺B220⁺NK细胞表面PDCA-1的表达存在差异。