Karimi-Khorrami Neda, Radi Mohsen, Amiri Sedigheh, Abedi Elahe, McClements David Julian
Department of Food Science and Technology, Yasuj Branch, Islamic Azad University, Yasuj, Iran.
Department of Food Science and Technology, Yasuj Branch, Islamic Azad University, Yasuj, Iran; Sustainable Agriculture and Food Security Research Group, Yasuj Branch, Islamic Azad University, Yasuj, Iran.
Int J Biol Macromol. 2022 May 15;207:801-812. doi: 10.1016/j.ijbiomac.2022.03.149. Epub 2022 Mar 28.
Antimicrobial biopolymer films were prepared by incorporating thymol-loaded nanostructured lipid carriers (NLC) or nanoemulsions (NE) into Ca-alginate solutions. Thymol-loaded-NLCs with thymol/lipid mass ratios of 0.1 and 0.2 were prepared and then used to fabricate NLC/alginate films containing either 20% (NLC20 film) or 10% (NLC10 film) of NLCs. Consequently, these two films had the same total thymol mass fraction: R = 0.02. A nanoemulsion-loaded film (NE film) containing the same amount of thymol and a neat alginate film (control) were also prepared. Incorporation of the NLCs increased the porosity and surface roughness, thickness, water vapor permeability, and yellowness of the films, but decreased their water contact angle, mechanical strength, and swelling ratio. The release of thymol into the air and into water-ethanol solutions was slower for NLC-loaded than NE-loaded films, moreover being slower for the NLC20 than NLC10 films. The antimicrobial activity of the active films was tested on ground beef samples. Their antimicrobial activity was correlated to their release rates, with the NLC20 film giving the longest protection against the enumerated microorganisms. Our results show that encapsulating antimicrobial essential oils within NLCs was more effective at creating antimicrobial films with sustained release properties than encapsulating them within NEs.
通过将负载百里香酚的纳米结构脂质载体（NLC）或纳米乳液（NE）掺入海藻酸钙溶液中来制备抗菌生物聚合物薄膜。制备了百里香酚与脂质质量比为0.1和0.2的负载百里香酚的NLC，然后用于制备含有20%（NLC20薄膜）或10%（NLC10薄膜）NLC的NLC/海藻酸盐薄膜。因此，这两种薄膜具有相同的总百里香酚质量分数：R = 0.02。还制备了含有相同量百里香酚的负载纳米乳液的薄膜（NE薄膜）和纯海藻酸盐薄膜（对照）。掺入NLC增加了薄膜的孔隙率、表面粗糙度、厚度、水蒸气透过率和黄度，但降低了它们的水接触角、机械强度和溶胀率。负载NLC的薄膜中百里香酚向空气和水 - 乙醇溶液中的释放比负载NE的薄膜慢，而且NLC20薄膜比NLC10薄膜更慢。在碎牛肉样品上测试了活性薄膜的抗菌活性。它们的抗菌活性与其释放速率相关，NLC20薄膜对所列微生物提供的保护时间最长。我们的结果表明，将抗菌精油封装在NLC中比将其封装在NE中更有效地制备具有缓释特性的抗菌薄膜。
