Favrot M C, Frappaz D, Maritaz O, Philip I, Fontaniere B, Gentilhomme O, Bailly C, Zucker J M, Gentet J C, Kemshead J
Br J Cancer. 1986 Oct;54(4):637-41. doi: 10.1038/bjc.1986.220.
On 80 occasions 4 iliac biopsy trephines and 4 iliac aspirations were performed in 37 children with neuroblastoma at various stages of the disease. In 38 of these procedures, tumour cells were detected. In 24% of cases, both trephines and aspirates were positive, whereas in 63% neuroblastoma cells were detected only on the trephines and in 13% only on the aspirates. In addition, in 37% of the stagings, only one out of the 8 investigations was abnormal. Only in one of 33 pathological cases, was BM involvement diagnosed on trephine imprint. No involvement was ever observed on tibial and sternal aspirates without iliac involvement. Immunological studies with two monoclonal antibodies HSAN 1-2 and UJ13A were performed on 56 occasions. Cytohistological and immunological studies were concordant in 39. In 3 studies, the antigens recognized by the two monoclonal antibodies were not expressed by the initial tumour and in 3 additional studies immunological results were falsely negative; but in 11 cases monoclonal antibodies identified residual malignancy despite normal cytohistology. From this study, biopsies appear more helpful to detect malignant cells than aspirates. Immunological staining clearly leads to a better definition of tumour cells in aspirates.
对37例处于疾病不同阶段的神经母细胞瘤患儿进行了80次操作，其中4次为髂骨活检环钻术，4次为髂骨穿刺术。在这些操作中，有38次检测到肿瘤细胞。在24%的病例中，环钻术和穿刺术均呈阳性，而在63%的病例中，仅在环钻术中检测到神经母细胞瘤细胞，13%的病例仅在穿刺术中检测到。此外，在37%的分期中，8项检查中只有1项异常。在33例病理病例中，只有1例通过环钻印片诊断出骨髓受累。在没有髂骨受累的情况下，从未在胫骨和胸骨穿刺中观察到受累情况。使用两种单克隆抗体HSAN 1-2和UJ13A进行了56次免疫学研究。细胞组织学和免疫学研究在39次中结果一致。在3次研究中，两种单克隆抗体识别的抗原在初始肿瘤中未表达，在另外3次研究中，免疫学结果为假阴性；但在11例病例中，尽管细胞组织学正常，单克隆抗体仍识别出残留恶性肿瘤。从这项研究来看，活检似乎比穿刺术在检测恶性细胞方面更有帮助。免疫染色显然能更好地界定穿刺物中的肿瘤细胞。
