Sun Zhe, Yu Tong, Cao Xiangyu, Gao Lili, Pang Qiuxiang, Liu Baohua, Deng Hongkuan
School of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255000, China.
School of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255000, China.
Gene. 2022 Jun 5;826:146464. doi: 10.1016/j.gene.2022.146464. Epub 2022 Mar 28.
Deoxyribonuclease II (DNase II) has been found to regulate inflammation, autoimmunity and apoptosis in vertebrates and invertebrates. The strong capacity of degrading DNA makes DNase II play an important role in the immune process. Planarian has become one of the model references due to its strong immune system, the environment they live makes planarians face the threat of microorganisms and injury, the strong immune system can protect planarians from the threat of bacterial and infection. In this study, we found that there was DNase in the lysis buffer of planarians, then we acquired the sequence of DjDN2s (Dugesia japonica DNase2s) and confirmed the DjDN2s were conserved DNase IIs. The predicted structure showed the active sites and binding patterns of DjDN2s. Whole-mount in situ hybridization results showed DjDN2s mainly expressed in immune organs. Quantitative real-time PCR revealed that the expression of DjDN2s upregulated in varying degrees when got hurt and challenged with bacteria, and the knockdown of DjDN2s led to the slower repair of wound. The recombinant phages which take DjDN2 also had the ability to degrade DNA and clear young biofilm of Gram-negative bacteria. Collectively, DNase II of planarian might play a role in the antimicrobial response and wound-induced response.
脱氧核糖核酸酶II（DNase II）已被发现在脊椎动物和无脊椎动物中调节炎症、自身免疫和细胞凋亡。其强大的DNA降解能力使DNase II在免疫过程中发挥重要作用。涡虫因其强大的免疫系统成为模型参考之一，它们生活的环境使涡虫面临微生物和损伤的威胁，强大的免疫系统可保护涡虫免受细菌和感染的威胁。在本研究中，我们发现涡虫裂解缓冲液中存在DNase，随后我们获得了日本三角涡虫DNase2（DjDN2s）的序列，并证实DjDN2s是保守的DNase II。预测结构显示了DjDN2s的活性位点和结合模式。整体原位杂交结果表明DjDN2s主要在免疫器官中表达。定量实时PCR显示，受伤和受到细菌攻击时，DjDN2s的表达不同程度上调，敲低DjDN2s导致伤口修复变慢。携带DjDN2的重组噬菌体也具有降解DNA和清除革兰氏阴性菌早期生物被膜的能力。总的来说，涡虫的DNase II可能在抗菌反应和伤口诱导反应中发挥作用。
