Division of Environmental Science and Ecological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
Division of Environmental Science and Ecological Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
J Hazard Mater. 2022 Jun 15;432:128755. doi: 10.1016/j.jhazmat.2022.128755. Epub 2022 Mar 23.
A novel method for the volumetric detection of microplastics in various environmental (soil, water) and food (fish, meat) matrices was developed. The method is based on the Nile Red staining of microplastics while eliminating probable interference by other organic polymers such as lignin, chitin, cellulosic materials, and other organic substances using a mixture of three water-based dyes (Calcofluor White, Evans Blue, and 4,6-diamidino-2-phenylindole [DAPI]). The excitation/emission 'sweet spot' was determined for water based blue dyes to detect them in a single channel for effective elimination of probable contaminations. Detection of microplastic particles using the Nile Red method was validated by comparing with traditional detection of microplastics via Fourier transform infrared spectroscopy (FTIR). Volumetric measurements of the microplastics present in environmental samples were made possible using Z-stack confocal microscopy images backed by threshold-based 3D segmentation. Regularly shaped microplastic materials were used to validate the volumetric measurement method. The proposed volumetric determination method will be very useful for screening microplastics in diverse media and improving the prevailing method using FTIR.
开发了一种新颖的方法，用于对各种环境（土壤、水）和食品（鱼、肉）基质中的微塑料进行体积检测。该方法基于尼罗红对微塑料进行染色，同时使用三种基于水的染料（Calcofluor White、Evans Blue 和 4,6-二脒基-2-苯基吲哚 [DAPI]）混合物消除木质素、几丁质、纤维素材料和其他有机物质等可能的有机聚合物的干扰。确定了基于水的蓝色染料的激发/发射“最佳点”，以便在单个通道中检测它们，从而有效消除可能的污染。通过与传统的傅里叶变换红外光谱（FTIR）检测微塑料的方法进行比较，验证了使用尼罗红方法检测微塑料的有效性。使用基于阈值的 3D 分割的 Z 堆叠共聚焦显微镜图像，实现了对环境样品中存在的微塑料的体积测量。使用规则形状的微塑料材料对体积测量方法进行了验证。该提出的体积测定方法对于筛选各种介质中的微塑料非常有用，并可以改进现有的使用 FTIR 的方法。
