Department of Toxicology and Bromatology, Faculty of Pharmacy, Collegium Medicum of Nicolaus Copernicus University, Bydgoszcz, Poland.
Department of Neurology, 10th Military Research Hospital and Polyclinic, Bydgoszcz, Poland.
Folia Neuropathol. 2022;60(1):122-127. doi: 10.5114/fn.2022.114053.
The search for new diagnostic and therapeutic approaches for myasthenia gravis (MG) is highly desirable. Therefore, the aim of the present study is to assess the profile of non-essential amino acids in the serum of MG patients. We evaluated the serum levels of non-essential amino acids in MG patients (n = 10) and control subjects (CS, n = 10) using high-performance liquid chromatography (HPLC) method and assuming a two-fold concentration difference between the groups as significant. Serum levels of aspartic acid and glutamic acid in MG patients were significantly higher than in CS. There were no significant differences in mean serum levels of glycine, proline, alanine, serine, cysteine, arginine and tyrosine between MG patients and CS. The results indicate the need for further research to assess the role of non-essential amino acids in MG. Moreover, our preliminary results suggest that the metabolism of some amino acids seems to be changed in patients with MG. Therefore, we conclude that amino acids profiling might be helpful in searching for putative biomarkers of the central nervous system diseases such as myasthenia gravis.
寻找重症肌无力 (MG) 的新诊断和治疗方法是非常需要的。因此，本研究旨在评估 MG 患者血清中非必需氨基酸的特征。我们使用高效液相色谱 (HPLC) 方法评估了 10 名 MG 患者和 10 名对照受试者 (CS) 的血清中非必需氨基酸水平，并假设两组之间浓度差异两倍为显著。MG 患者的血清天门冬氨酸和谷氨酸水平明显高于 CS。MG 患者与 CS 之间的甘氨酸、脯氨酸、丙氨酸、丝氨酸、半胱氨酸、精氨酸和酪氨酸的平均血清水平无显著差异。结果表明需要进一步研究来评估非必需氨基酸在 MG 中的作用。此外，我们的初步结果表明，MG 患者的一些氨基酸代谢似乎发生了变化。因此，我们得出结论，氨基酸谱分析可能有助于寻找重症肌无力等中枢神经系统疾病的潜在生物标志物。
