收集和培养苔藓动物用于再生研究。
Collecting and Culturing Bryozoans for Regenerative Studies.
机构信息
Department of Marine Science, University of Otago, Dunedin, New Zealand.
出版信息
Methods Mol Biol. 2022;2450:151-177. doi: 10.1007/978-1-0716-2172-1_8.
Abstract
Among marine invertebrates, bryozoans are small, not well known, and complex to identify. Nevertheless, they offer unique opportunities for whole-body generation research, because of their colonial, modular mode of growth. Here, we describe detailed methods for collection of bryozoans from a range of environments, sample preparation and identification, culture and feeding, spawning and breeding, marking colonies for growth studies, and histological preparation.
摘要
在海洋无脊椎动物中，苔藓动物体型较小，鲜为人知，且难以识别。然而，由于其具有群体、模块化的生长方式，它们为整体生物发生研究提供了独特的机会。在这里，我们描述了从各种环境中收集苔藓动物、样品准备和鉴定、培养和饲养、产卵和繁殖、标记用于生长研究的群体以及组织学准备的详细方法。
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