在一种新型模式生物——淡水绿色长吻沙蚕中研究环节动物再生
Studying Annelida Regeneration in a Novel Model Organism: The Freshwater Aeolosoma viride.
作者信息
Chen Chiao-Ping, Fok Sheridan Ke-Wing, Chen Cheng-Yi, Hsu Fei-Man, Hsieh Yu-Wen, Chen Jiun-Hong
机构信息
Department of Life Science, National Taiwan University, Taipei, Taiwan.
出版信息
Methods Mol Biol. 2022;2450:179-194. doi: 10.1007/978-1-0716-2172-1_9.
Abstract
Aeolosoma viride, a globally distributed freshwater annelid, has a semitransparent appearance with 10 to 12 segments, about 2 to 3 mm in length. It is easy to raise and handle in laboratory conditions. Due to its robust regenerative capacity and applicability of various molecular tools including EdU labeling, whole-mount in situ hybridization (WISH), and RNA interference (RNAi), it rises as a promising model for studying whole-body regeneration.
摘要
绿色颤蚓是一种分布于全球的淡水环节动物，外观半透明，有10至12节，体长约2至3毫米。在实验室条件下易于饲养和操作。由于其强大的再生能力以及包括EdU标记、全组织原位杂交（WISH）和RNA干扰（RNAi）在内的各种分子工具的适用性，它成为研究全身再生的一个有前景的模型。
相似文献
1
Studying Annelida Regeneration in a Novel Model Organism: The Freshwater Aeolosoma viride.在一种新型模式生物——淡水绿色长吻沙蚕中研究环节动物再生
Methods Mol Biol. 2022;2450:179-194. doi: 10.1007/978-1-0716-2172-1_9.
2
Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride.淡水环节动物 Aeolosoma viride 中再生和衰老过程中的端粒维持。
Sci Rep. 2018 Dec 24;8(1):18078. doi: 10.1038/s41598-018-36396-y.
3
Caspase dependent apoptosis is required for anterior regeneration in Aeolosoma viride and its related gene expressions are regulated by the Wnt signaling pathway.依赖半胱氨酸天冬氨酸蛋白酶的细胞凋亡对于绿蝾螈的前部再生是必需的，其相关基因的表达受 Wnt 信号通路的调控。
Sci Rep. 2020 Jul 1;10(1):10692. doi: 10.1038/s41598-020-64008-1.
4
A new species of the genus Aeolosoma (Annelida: Aphanoneura: Aeolosomatidae) with peculiar sigmoid chaetae from fresh waters of Vietnam.越南淡水生境中具独特“S”形刚毛的 Aeolosoma 属（环节动物门：无刚毛纲： Aeolosomatidae）一新种。
Zootaxa. 2021 Nov 30;5072(3):285-290. doi: 10.11646/zootaxa.5072.3.5.
5
First report on the chromosomes of Aeolosoma viride and Aeolosoma hemprichi (Aeolosomatidae; Annelida).关于绿色颤蚓和亨氏颤蚓（颤蚓科；环节动物门）染色体的首次报告。
Zoolog Sci. 2008 Sep;25(9):904-6. doi: 10.2108/zsj.25.904.
6
Whole-Mount In Situ Hybridization of Planarians.涡虫的整体原位杂交
Methods Mol Biol. 2018;1774:379-392. doi: 10.1007/978-1-4939-7802-1_12.
7
Novel monoclonal antibodies to study tissue regeneration in planarians.用于研究涡虫组织再生的新型单克隆抗体。
BMC Dev Biol. 2015 Jan 21;15:2. doi: 10.1186/s12861-014-0050-9.
8
Formaldehyde-based whole-mount in situ hybridization method for planarians.涡虫基于甲醛的全胚胎原位杂交方法
Dev Dyn. 2009 Feb;238(2):443-50. doi: 10.1002/dvdy.21849.
9
Ingestion of bacterially expressed double-stranded RNA inhibits gene expression in planarians.摄入细菌表达的双链RNA会抑制涡虫中的基因表达。
Proc Natl Acad Sci U S A. 2003 Sep 30;100 Suppl 1(Suppl 1):11861-5. doi: 10.1073/pnas.1834205100. Epub 2003 Aug 13.
10
Whole-mount in situ hybridization using DIG-labeled probes in planarian.使用地高辛标记探针在涡虫中进行整体原位杂交。
Methods Mol Biol. 2014;1211:41-51. doi: 10.1007/978-1-4939-1459-3_4.
本文引用的文献
1
Caspase dependent apoptosis is required for anterior regeneration in Aeolosoma viride and its related gene expressions are regulated by the Wnt signaling pathway.依赖半胱氨酸天冬氨酸蛋白酶的细胞凋亡对于绿蝾螈的前部再生是必需的，其相关基因的表达受 Wnt 信号通路的调控。
Sci Rep. 2020 Jul 1;10(1):10692. doi: 10.1038/s41598-020-64008-1.
2
Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride.淡水环节动物 Aeolosoma viride 中再生和衰老过程中的端粒维持。
Sci Rep. 2018 Dec 24;8(1):18078. doi: 10.1038/s41598-018-36396-y.
3
Morphological, cellular and molecular characterization of posterior regeneration in the marine annelid Platynereis dumerilii.海洋多毛纲动物杜氏阔沙蚕尾部再生的形态学、细胞及分子特征
Dev Biol. 2019 Jan 15;445(2):189-210. doi: 10.1016/j.ydbio.2018.11.004. Epub 2018 Nov 13.
4
Developmental and molecular biology of annelid regeneration: a comparative review of recent studies.环节动物再生的发育与分子生物学：近期研究的比较综述
Curr Opin Genet Dev. 2016 Oct;40:144-153. doi: 10.1016/j.gde.2016.07.010. Epub 2016 Aug 6.
5
Latent regeneration abilities persist following recent evolutionary loss in asexual annelids.最近的进化研究表明，无性别环节动物失去有性生殖能力后，其潜在的再生能力仍然存在。
Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1464-9. doi: 10.1073/pnas.0907931107. Epub 2009 Dec 4.
6
Phylogeny of Annelida (Lophotrochozoa): total-evidence analysis of morphology and six genes.环节动物门（触手冠动物）的系统发育：形态学与六个基因的全证据分析
BMC Evol Biol. 2009 Aug 6;9:189. doi: 10.1186/1471-2148-9-189.
7
Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodies.使用5-乙炔基-2'-脱氧尿苷掺入并结合点击化学检测S期细胞周期进程，这是一种替代使用5-溴-2'-脱氧尿苷抗体的方法。
Biotechniques. 2008 Jun;44(7):927-9. doi: 10.2144/000112812.
8
A chemical method for fast and sensitive detection of DNA synthesis in vivo.一种用于体内DNA合成快速灵敏检测的化学方法。
Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2415-20. doi: 10.1073/pnas.0712168105. Epub 2008 Feb 12.
9
Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication.抗5-溴脱氧尿苷和5-碘脱氧尿苷单克隆抗体：一种检测DNA复制的新型试剂。
Science. 1982 Oct 29;218(4571):474-5. doi: 10.1126/science.7123245.
Zotero 插件