Centre for Environmental and Marine Studies (CESAM) & Department of Biology, University of Aveiro, Aveiro, Portugal.
Methods Mol Biol. 2022;2450:195-206. doi: 10.1007/978-1-0716-2172-1_10.
The polychaete Diopatra neapolitana is a cosmopolitan annelid that can robustly regenerate both its anterior and posterior body part depending on the position of the amputation. Previous studies demonstrated that body regeneration represents a sensitive and unspecific response to environmental stresses, including contaminants and climate alterations.The posterior body regeneration of D. neapolitana is thus a suitable, ecological and relevant biomarker in ecotoxicological and ecological risk assessment assays. Here we describe the amputation process, the monitoring of the regeneration process of the polychaete D. neapolitana and the quantification of the impact of environmental stresses on its regenerative capacity.
多毛类海洋生物 Diopatra neapolitana 是一种世界性分布的环节动物,能够根据截断位置强壮地再生其前体和后体部分。先前的研究表明,身体再生是对环境压力(包括污染物和气候变化)的敏感和非特异性反应。因此,D. neapolitana 的后体再生是生态毒理学和生态风险评估试验中合适的、生态相关的生物标志物。在这里,我们描述了多毛类海洋生物 D. neapolitana 的截断过程、再生过程的监测以及环境压力对其再生能力影响的定量分析。