Yokoi Hidenori, Matsumoto Yuma, Kawada Michitsugu, Sakurai Hiroyuki, Saito Koichiro
Department of Otolaryngology, Head and Neck Surgery, Kyorin University School of Medicine, Tokyo Japan.
Department of Pharmacology, Kyorin University School of Medicine, Tokyo Japan.
Allergy Rhinol (Providence). 2022 Mar 24;13:21526575221079260. doi: 10.1177/21526575221079260. eCollection 2022 Jan-Dec.
Allergic rhinitis is a typical type I hypersensitivity reaction, commonly caused by inhalant allergens. Accurate identification of the causative antigen is important for rapid diagnosis and treatment initiation.
This study examined the efficiency of serum-based allergen-specific immunoglobulin E and total immunoglobulin E antibody titers in screening for pollen allergy. We also examined the effect of cross-reactive carbohydrate determinants on specific immunoglobulin E titers in screening for pollen allergy, one of the causes of false positivity in specific immunoglobulin E measurements.
A questionnaire was used to evaluate the symptoms of pollinosis among participants who underwent a medical examination. One hundred and thirty-two participants reported pollen allergy symptoms and 127 reported an absence of symptoms. Specific immunoglobulin E levels were measured using the AlaSTAT 3g Allergy method. Seventeen components, including four types of cross-reactive carbohydrate determinant-specific immunoglobulin E antibodies, were measured and evaluated comparatively.
The sensitivity and specificity of the tests in predicting the presence or absence of pollen allergy were analyzed. The values of the areas under the curves for immunoglobulin E antibody levels against cedar, cypress, orchard grass, and ragweed pollen were 0.87, 0.82, 0.63, and 0.56, respectively. A cross-reactive carbohydrate determinant-related false-positive effect on the pollen specific immunoglobulin E titer was noted in pollen screening.
Cedar pollen-specific immunoglobulin E titers showed sufficient accuracy for use in pollen allergy screening. The study of cross-reactive carbohydrate determinants suggested that subjects who tested positive for pollen often had false-positive results due to the impact of cross-reactive carbohydrate determinants.
变应性鼻炎是一种典型的I型超敏反应，通常由吸入性变应原引起。准确识别致病抗原对于快速诊断和开始治疗很重要。
本研究检测了基于血清的变应原特异性免疫球蛋白E和总免疫球蛋白E抗体滴度在筛查花粉过敏中的效率。我们还研究了交叉反应性碳水化合物决定簇对花粉过敏筛查中特异性免疫球蛋白E滴度的影响，交叉反应性碳水化合物决定簇是特异性免疫球蛋白E测量中假阳性的原因之一。
使用问卷评估接受体检的参与者的花粉症症状。132名参与者报告有花粉过敏症状，127名报告无症状。采用AlaSTAT 3g变应原检测方法测量特异性免疫球蛋白E水平。测量并比较了17种成分，包括4种交叉反应性碳水化合物决定簇特异性免疫球蛋白E抗体。
分析了检测在预测花粉过敏存在与否方面的敏感性和特异性。针对雪松、柏树、果园草和豚草花粉的免疫球蛋白E抗体水平的曲线下面积值分别为0.87、0.82、0.63和0.56。在花粉筛查中发现交叉反应性碳水化合物决定簇对花粉特异性免疫球蛋白E滴度有假阳性影响。
雪松花粉特异性免疫球蛋白E滴度在花粉过敏筛查中显示出足够的准确性。对交叉反应性碳水化合物决定簇的研究表明，花粉检测呈阳性的受试者由于交叉反应性碳水化合物决定簇的影响，往往有假阳性结果。
