Hemdan Dalia K, Selim Manar A, Galhom Rania A, El Daharawy Mohamed H, Hassan Hayam Y
Department of Endodontics, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
Department of Oral Biology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
J Oral Biol Craniofac Res. 2022 Mar-Apr;12(2):293-298. doi: 10.1016/j.jobcr.2022.03.009. Epub 2022 Mar 18.
This study evaluated exposed dogs' dental pulp response after direct capping with autologous dental pulp stem cells (DPSCs) and compared the results with MTA (Angelus, Brazil). Sixty teeth from six mongrel dogs were included (n = 60), allocated into four groups; negative control (n = 6), positive control (n = 6), MTA (n = 24), and DPSCs (n = 24). DPSCs were isolated and cultured from extracted first premolars (1premolar/dog). The teeth were prepared for histological and immunohistochemical analyses. Statistical analysis was calculated using ANOVA tests, P ≤ 0.05. MTA induced the formation of an almost complete calcific bridge. DPSCs successfully regenerated the injured pulp and dentin. The comparison between the capping groups and time intervals had a statistically significant effect, as well as, the interaction between the two variables. DPSCs had greater ability in the reparative and regenerative process of dentin and odontoblastic differentiation by having a significantly stronger TGFβ1 expression than MTA.
本研究评估了自体牙髓干细胞（DPSCs）直接盖髓后受试犬牙髓的反应，并将结果与MTA（巴西安吉利斯公司）进行比较。纳入了6只杂种犬的60颗牙齿（n = 60），分为四组：阴性对照组（n = 6）、阳性对照组（n = 6）、MTA组（n = 24）和DPSCs组（n = 24）。从拔除的第一前磨牙（每只犬1颗前磨牙）中分离并培养DPSCs。对牙齿进行组织学和免疫组织化学分析。采用方差分析进行统计分析，P≤0.05。MTA诱导形成了几乎完整的钙化桥。DPSCs成功地使损伤的牙髓和牙本质再生。盖髓组与时间间隔之间的比较具有统计学意义，以及这两个变量之间的相互作用。DPSCs在牙本质修复和再生过程以及成牙本质细胞分化方面具有更强的能力，因为其TGFβ1表达明显强于MTA。
