常规脱钙方法与骨和牙齿微波脱钙的比较。
Comparison of routine decalcification methods with microwave decalcification of bone and teeth.
作者信息
Sangeetha R, Uma K, Chandavarkar Vidya
机构信息
Department of Oral and Maxillofacial Pathology, Karnatak Lingayat Education Society's Institute of Dental Sciences, Bangalore, Karnataka, India.
Department of Oral and Maxillofacial Pathology, School of Dental Sciences, Sharada University, Greater Noida, Uttar Pradesh, India.
出版信息
J Oral Maxillofac Pathol. 2013 Sep;17(3):386-91. doi: 10.4103/0973-029X.125204.
INTRODUCTION
The microwave oven has been used quite often for tissue processing, but there are very few studies describing its use in decalcification of bone or teeth. In this study we have attempted to decalcify bone and teeth using a microwave oven and compare the process and results with conventional decalcification methods.
AIMS AND OBJECTIVES
The objectives of the study were to determine and compare routine decalcification with microwave decalcification of bone and teeth using 5% nitric acid, 5% formic acid, and 14% ethylenediaminetetraacetic acid (EDTA) with respect to speed of decalcification, preservation of tissue structure and staining efficacy.
MATERIALS AND METHODS
In our study the total sample size used for both routine and microwave decalcification was 30 premolar teeth and 30 pieces of condyles. The three solutions were dilute nitric acid (5%), formic acid (5%), and EDTA (14%). Each set consisting of the same type of premolars and condyles in each of the three decalcifying solutions were used in both manual method and microwave method.
RESULTS
The results in the present study confirmed the fact that the microwave method using nitric acid was indeed the fastest decalcifying method needing just about 2 days for condyle and 4 days for premolars, compared with routine decalcification. The results also showed that the overall histological picture was good with EDTA and formic acid irrespective of the methods used. In the routine method, nitric acid gave poor cellular detail when compared with microwave method.
CONCLUSION
With our study we conclude that microwave oven decalcification is faster than routine decalcification irrespective of the decalcifying agents used. The tissue preservation and staining efficacy was good in microwave nitric acid decalcification compared to routine nitric acid decalcification. Both formic acid and EDTA show good tissue preservation and staining efficacy irrespective of the method used.
引言
微波炉已被频繁用于组织处理，但很少有研究描述其在骨骼或牙齿脱钙中的应用。在本研究中，我们尝试使用微波炉对骨骼和牙齿进行脱钙，并将该过程及结果与传统脱钙方法进行比较。
目的
本研究的目的是使用5%硝酸、5%甲酸和14%乙二胺四乙酸（EDTA），就脱钙速度、组织结构保存和染色效果，确定并比较骨骼和牙齿的常规脱钙与微波脱钙。
材料与方法
在我们的研究中，用于常规脱钙和微波脱钙的样本总数为30颗前磨牙和30块髁突。三种溶液分别为稀硝酸（5%）、甲酸（5%）和EDTA（14%）。在手动方法和微波方法中，每组均使用三种脱钙溶液中相同类型的前磨牙和髁突。
结果
本研究结果证实，与常规脱钙相比，使用硝酸的微波方法确实是最快的脱钙方法，髁突只需约2天，前磨牙只需4天。结果还表明，无论使用何种方法，EDTA和甲酸处理后的整体组织学图像都很好。在常规方法中，与微波方法相比，硝酸处理后的细胞细节较差。
结论
通过我们的研究得出结论，无论使用何种脱钙剂，微波炉脱钙都比常规脱钙更快。与常规硝酸脱钙相比，微波硝酸脱钙在组织保存和染色效果方面表现良好。无论使用何种方法，甲酸和EDTA都显示出良好的组织保存和染色效果。
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