Feng Yuan, Luo Jing, Cheng Jintao, Xu Aimin, Qiu Dongbo, He Sixiao, Zheng Dayong, Jia Changchang, Zhang Qi, Lin Nan
Department of Hepatobiliary Surgery, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
Cell-gene Therapy Translational Medicine Research Center, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
Front Bioeng Biotechnol. 2022 Mar 14;10:819148. doi: 10.3389/fbioe.2022.819148. eCollection 2022.
Mesenchymal stem cells (MSCs) have a variety of unique properties, such as stem cell multipotency and immune regulation, making them attractive for use in cell therapy. Before infusion therapy, MSCs are required to undergo tissue separation, purification, and expansion for a certain duration. During the process of expansion of MSCs, the influence of culture time and environment can lead to cell senescence, increased heterogeneity, and function attenuation, which limits their clinical applications. We used a cocktail of three small-molecule compounds, ACY (A-83-01, CHIR99021, and Y-27632), to increase the proliferation activity of MSCs and reduce cell senescence. ACY inhibited the increase in heterogeneity of MSCs and conserved their differentiation potential. Additionally, ACY maintained the phenotype of MSCs and upregulated the expression of immunomodulatory factors. These results suggest that ACY can effectively improve the quantity and quality of MSCs.
间充质干细胞（MSCs）具有多种独特特性，如干细胞多能性和免疫调节能力，这使其在细胞治疗中具有吸引力。在进行输注治疗之前，MSCs需要经过组织分离、纯化，并在一定时间内进行扩增。在MSCs的扩增过程中，培养时间和环境的影响会导致细胞衰老、异质性增加和功能衰减，这限制了它们的临床应用。我们使用了三种小分子化合物组成的混合物ACY（A-83-01、CHIR99021和Y-27632）来提高MSCs的增殖活性并减少细胞衰老。ACY抑制了MSCs异质性的增加，并保留了它们的分化潜能。此外，ACY维持了MSCs的表型，并上调了免疫调节因子的表达。这些结果表明，ACY可以有效地提高MSCs的数量和质量。
