Wang Qiao, Song Wanying, Tian Yimei, Hu Peihao, Liu Xin, Xu Lin, Gong Zhiyong
Key Laboratory for Deep Processing of Major Grain and Oil (The Chinese Ministry of Education), College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China.
Front Nutr. 2022 Mar 14;9:837601. doi: 10.3389/fnut.2022.837601. eCollection 2022.
Perchlorate, commonly available in drinking water and food, acts on the iodine uptake by the thyroid affecting lipid metabolism. High-fat diets leading to various health problems continually raise public concern. In the present study, liver lipid metabolism profiles and metabolic pathways were investigated in C57BL/6J mice chronically exposed to perchlorate using targeted metabolomics. Mice were fed a high-fat diet and treated orally with perchlorate at 0.1 mg/kg bw (body weight), 1 mg/kg bw and 10 mg/kg bw daily for 12 weeks. Perchlorate induced disorders of lipid metabolism and hepatic lipid accumulation confirmed by serum biochemical parameters and histopathological examination. There were 34 kinds of lipid in liver detected by UHPLC-MS/MS and key metabolites were identified by multivariate statistical analysis evaluated with VIP > 1, -value < 0.05, fold change > 1.2 or < 0.8. Perchlorate low, medium and high dose groups were identified with 11, 7 and 8 significantly altered lipid metabolites compared to the control group, respectively. The results of the metabolic pathway analysis revealed that the differential metabolites classified into different experimental groups contribute to the glycerophospholipid metabolic pathway. These findings provide insights into the effects of perchlorate on lipid metabolism during long-term exposure to high-fat diets and contribute to the evaluation of perchlorate liver toxic mechanisms and health effects.
高氯酸盐常见于饮用水和食物中，它作用于甲状腺对碘的摄取，影响脂质代谢。导致各种健康问题的高脂饮食不断引发公众关注。在本研究中，使用靶向代谢组学方法，对长期暴露于高氯酸盐的C57BL/6J小鼠的肝脏脂质代谢谱和代谢途径进行了研究。给小鼠喂食高脂饮食，并每天以0.1毫克/千克体重、1毫克/千克体重和10毫克/千克体重的剂量口服高氯酸盐，持续12周。血清生化参数和组织病理学检查证实，高氯酸盐会导致脂质代谢紊乱和肝脏脂质蓄积。通过超高效液相色谱-串联质谱法（UHPLC-MS/MS）在肝脏中检测到34种脂质，并通过多变量统计分析确定关键代谢物，其中变量重要性投影（VIP）>1、P值<0.05、变化倍数>1.2或<0.8。与对照组相比，低、中、高剂量高氯酸盐组分别鉴定出11种、7种和8种显著改变的脂质代谢物。代谢途径分析结果表明，不同实验组中分类的差异代谢物参与甘油磷脂代谢途径。这些发现为长期暴露于高脂饮食期间高氯酸盐对脂质代谢的影响提供了见解，并有助于评估高氯酸盐的肝脏毒性机制和健康影响。
