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用于翻译后蛋白质变体细胞成像的分子探针。

Molecular probes for cellular imaging of post-translational proteoforms.

作者信息

Suraritdechachai Surased, Lakkanasirorat Benya, Uttamapinant Chayasith

机构信息

School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC) Rayong Thailand

出版信息

RSC Chem Biol. 2022 Jan 4;3(2):201-219. doi: 10.1039/d1cb00190f. eCollection 2022 Feb 9.

DOI:10.1039/d1cb00190f
PMID:35360891
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8826509/
Abstract

Specific post-translational modification (PTM) states of a protein affect its property and function; understanding their dynamics in cells would provide deep insight into diverse signaling pathways and biological processes. However, it is not trivial to visualize post-translational modifications in a protein- and site-specific manner, especially in a living-cell context. Herein, we review recent advances in the development of molecular imaging tools to detect diverse classes of post-translational proteoforms in individual cells, and their applications in studying precise roles of PTMs in regulating the function of cellular proteins.

摘要

蛋白质的特定翻译后修饰（PTM）状态会影响其性质和功能；了解它们在细胞中的动态变化将为深入了解各种信号通路和生物学过程提供深刻见解。然而，以蛋白质和位点特异性的方式可视化翻译后修饰并非易事，尤其是在活细胞环境中。在此，我们综述了分子成像工具开发方面的最新进展，这些工具可用于检测单个细胞中不同类型的翻译后蛋白变体，以及它们在研究PTM在调节细胞蛋白功能中的精确作用方面的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/634f/8826509/d307f36fa391/d1cb00190f-p3.jpg
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本文引用的文献

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Spatial mapping of protein composition and tissue organization: a primer for multiplexed antibody-based imaging.蛋白质组成和组织架构的空间定位：基于多重抗体成像的入门指南。
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