Olivereau M, Dubourg P, Chambolle P, Olivereau J
Cell Tissue Res. 1986;246(2):425-37. doi: 10.1007/BF00215905.
Male silver eels were injected with estradiol-17 beta (E2) to induce the development of gonadotropic (GTH) cells. They were subsequently injected with luteinizing hormone-releasing hormone (LHRH). Exocytotic figures and the lysis of some large globules and granules were observed. Morphometric studies showed a significant increase in the percentage of vacuoles after 4 and 6 injections of LHRH and a slight but significant decrease of granules. This response did not, however, occur in all GTH cells which never appeared completely degranulated and did not reach a vesicular stage. Hemi-pituitaries of E2-pretreated eels were incubated with or without LHRH (20 min to 2 h). Although typical exocytoses were not detected, an increased number of small granules near the basal lamina and lytic processes (globules with a raspberry-shaped structure, granules with variable electron density) were observed in the LHRH-incubated hemi-pituitaries compared with those kept in a control medium. The structure of GTH cells and their response to LHRH has been studied only under conditions of artificial stimulation, and their functional similarity to GTH cells of spontaneously maturing eels is discussed. Large female eels had unstimulated GTH cells. Growth hormone (STH), thyrotropic (TSH) and prolactin (PRL) cells were stimulated after E2 and LHRH. As with GTH cells, they regressed slowly after treatment was discontinued.
给雄性银鳗注射17β -雌二醇（E2）以诱导促性腺激素（GTH）细胞的发育。随后给它们注射促黄体生成素释放激素（LHRH）。观察到了胞吐现象以及一些大的球状物和颗粒的溶解。形态计量学研究表明，在注射4次和6次LHRH后，液泡百分比显著增加，颗粒略有但显著减少。然而，这种反应并非在所有GTH细胞中都出现，这些细胞从未完全脱颗粒，也未达到囊泡阶段。将经E2预处理的鳗鱼的半脑垂体在有或无LHRH（20分钟至2小时）的情况下进行孵育。虽然未检测到典型的胞吐作用，但与保存在对照培养基中的半脑垂体相比，在经LHRH孵育的半脑垂体中观察到基膜附近小颗粒数量增加以及溶解过程（具有覆盆子状结构的球状物、电子密度可变的颗粒）。仅在人工刺激条件下研究了GTH细胞的结构及其对LHRH的反应，并讨论了它们与自然成熟鳗鱼的GTH细胞的功能相似性。大型雌性鳗鱼的GTH细胞未受刺激。在注射E2和LHRH后，生长激素（STH）、促甲状腺激素（TSH）和催乳素（PRL）细胞受到刺激。与GTH细胞一样，在停止治疗后它们恢复缓慢。
