Dubourg P, Burzawa-Gerard E, Chambolle P, Kah O
Gen Comp Endocrinol. 1985 Sep;59(3):472-81. doi: 10.1016/0016-6480(85)90407-1.
Immunocytochemical techniques were used at the light and electron microscopical levels in order to localize and to characterize the gonadotrophs in the goldfish pituitary gland by means of antibodies to carp gonadotrophin (c-GTH) or its subunit (c-GTH beta). At the light microscopical level antibodies to c-GTH reacted weakly with cells located in the rostral pars distalis (RPD) and strongly with cells of the proximal pars distalis (PPD). The labeling was restricted to the proximal pars distalis when antibodies to c-GTH beta were employed. The PAP and colloidal-gold postembedding procedures demonstrated that two cell types of the PPD react with both immune sera. These cells correspond to the so-called globular and nonglobular basophils of the goldfish pituitary. The labeling was located over the small secretory granules and the large globules. A relationship was noted between the intensity of the labeling and the electron density of the globules.
为了通过针对鲤鱼促性腺激素（c-GTH）或其亚基（c-GTHβ）的抗体在金鱼脑垂体中定位并表征促性腺激素细胞，在光学和电子显微镜水平上使用了免疫细胞化学技术。在光学显微镜水平上，针对c-GTH的抗体与位于吻端远侧部（RPD）的细胞反应较弱，而与近侧部远侧部（PPD）的细胞反应强烈。当使用针对c-GTHβ的抗体时，标记仅限于近侧部远侧部。PAP和胶体金包埋后程序表明，PPD的两种细胞类型都与两种免疫血清反应。这些细胞对应于金鱼脑垂体中所谓的球状和非球状嗜碱性粒细胞。标记位于小分泌颗粒和大球体上。标记强度与球体的电子密度之间存在关联。
