恒河猴α1-抗胰蛋白酶异质性的研究。

Investigation of the heterogeneity of rhesus monkey alpha 1-antitrypsin.

作者信息

Berninger R W, Teixeira M F

出版信息

Comp Biochem Physiol B. 1986;85(2):325-32. doi: 10.1016/0305-0491(86)90007-6.

Abstract

Rhesus monkey alpha 1-antitrypsin (n = 144) was examined for heterogeneity by acid starch gel electrophoresis, isoelectric focusing in agarose and agarose gel electrophoresis. In contrast to other studies, no heterogeneity of Rhesus monkey alpha 1-antitrypsin could be documented using specific antisera. Rhesus monkey alpha 1-antitrypsin contained a reactive thiol. The pIs of the major isoforms of Rhesus monkey alpha 1-antitrypsin were 4.63, 4.69, 4.84 and 4.86 at 4 degrees C. No deficiency state of Rhesus monkey alpha 1-antitrypsin was detected. The six protease inhibitors in Rhesus monkey sera cross-reacted with antisera to the six human protease inhibitors.

摘要

通过酸性淀粉凝胶电泳、琼脂糖等电聚焦和琼脂糖凝胶电泳对恒河猴α1-抗胰蛋白酶（n = 144）进行了异质性检测。与其他研究不同，使用特异性抗血清未发现恒河猴α1-抗胰蛋白酶存在异质性。恒河猴α1-抗胰蛋白酶含有一个反应性巯基。在4℃时，恒河猴α1-抗胰蛋白酶主要同工型的等电点分别为4.63、4.69、4.84和4.86。未检测到恒河猴α1-抗胰蛋白酶缺乏状态。恒河猴血清中的六种蛋白酶抑制剂与针对六种人类蛋白酶抑制剂的抗血清发生交叉反应。