α1 -抗胰蛋白酶在酸性淀粉凝胶电泳和等电聚焦上的PI模式比较。

Comparison of the PI patterns of alpha 1-antitrypsin on acid starch gel electrophoresis and isoelectric focusing.

作者信息

Frants R R, Hooghwinkel G J, Eriksson A W

出版信息

Scand J Clin Lab Invest. 1981 Feb;41(1):15-20. doi: 10.3109/00365518109092009.

DOI:10.3109/00365518109092009

Abstract

An acid starch gel electrophoresis procedure was developed for recognition of PI M subtypes of alpha 1-antitrypsin, proving that their electrophoretic mobilities correspond to their relative isoelectric points on isoelectric focusing. From transfer experiments, it could be concluded that the PI patterns obtained after acid starch gel electrophoresis and isoelectric focusing are formed by identical molecular populations. These findings justify the replacement of acid starch gel electrophoresis by isoelectric focusing as the method of choice for typing, including the assignment of new PI variants.

摘要

开发了一种酸性淀粉凝胶电泳方法，用于识别α1-抗胰蛋白酶的PI M亚型，证明其电泳迁移率与等电聚焦时的相对等电点相对应。从转移实验可以得出结论，酸性淀粉凝胶电泳和等电聚焦后获得的PI模式是由相同的分子群体形成的。这些发现证明用等电聚焦取代酸性淀粉凝胶电泳作为分型的首选方法是合理的，包括新PI变体的鉴定。