School and Hospital of Stomatology, Shanxi Medical University, Taiyuan, Shanxi, China.
Eur Rev Med Pharmacol Sci. 2022 Mar;26(6):1816-1824. doi: 10.26355/eurrev_202203_28325.
Dental pulp stem cells (DPSCs) are adult stem cells of neural crest origin, are readily available, have good self-renewal and multidirectional differentiation properties, can differentiate into a variety of cells, are abundant, less harmful to donate, have no ethical issues, low immunogenicity, and therefore, are widely used as seed cells in the field of tissue engineering and regenerative medicine. MicroRNA (miRNA) is a single-stranded non-coding small RNA consisting of about 22 nucleotides, which plays an important regulatory role in various aspects of cellular activities, such as proliferation, differentiation, and apoptosis. In this paper, we review the regulatory role of small RNA in the differentiation of DPSCs and its mechanism in the past 5 years. This paper aims to reveal the important role of miRNAs in differentiation in DPSCs.
MicroRNAs (miRNAs), differentiation, and DPSCs were extensively searched in three databases from 2014 to 2021. These databases include PubMed, Cochrane Library, Embase.
Our study reviews the microRNAs (miR-145, miR-143-3p, miR-140-5p, miR-488, miR-218, miR-125a-3p, miR-27a-5p, miR-223, miR-21, miR-143, miR-215, miR-219a-1-3p, miR-31, miR-496, miR-218, miR-24-3p, miR-146a-5p, miR-196a, miR-188-3p, miR-424, miR-378a, miR-135, miR-124) in the differentiation of DPSCs.
A large body of evidence supports the involvement of miRNAs in differentiation associated with mesenchymal stem cells (MSCs), although the mechanisms involved are not yet clear. Most of the current studies are from in vitro studies, but the ultimate goal is to apply these studies to the clinic, and studies involving in vivo models are needed.
牙髓干细胞（DPSCs）是神经嵴来源的成体干细胞，易于获取，具有良好的自我更新和多向分化特性，能分化为多种细胞，丰富、供体伤害较小、无伦理问题、免疫原性低，因此广泛应用于组织工程和再生医学领域的种子细胞。微小 RNA（miRNA）是一种由约 22 个核苷酸组成的单链非编码小分子 RNA，在细胞活动的增殖、分化和凋亡等各个方面发挥重要的调控作用。本文综述了近 5 年来小 RNA 对 DPSCs 分化的调控作用及其机制。旨在揭示 miRNAs 在 DPSCs 分化中的重要作用。
在 2014 年至 2021 年期间，我们在 3 个数据库（PubMed、Cochrane Library、Embase）中广泛搜索了微小 RNA（miRNAs）、分化和 DPSCs。
我们的研究综述了微小 RNA（miR-145、miR-143-3p、miR-140-5p、miR-488、miR-218、miR-125a-3p、miR-27a-5p、miR-223、miR-21、miR-143、miR-215、miR-219a-1-3p、miR-31、miR-496、miR-218、miR-24-3p、miR-146a-5p、miR-196a、miR-188-3p、miR-424、miR-378a、miR-135、miR-124）在 DPSCs 分化中的作用。
大量证据表明微小 RNA 参与了间充质干细胞（MSCs）分化相关的过程，尽管涉及的机制尚不清楚。目前的大多数研究来自于体外研究，但最终目标是将这些研究应用于临床，需要涉及体内模型的研究。
