Bassett Charlton, Triplett Hunter, Lott Keegan, Howard Katherine M, Kingsley Karl
School of Medicine, University of Nevada, Las Vegas 1700 West Charleston Boulevard, Las Vegas, NV 89106, USA.
School of Dental Medicine, University of Nevada, Las Vegas 1001 Shadow Lane, Las Vegas, NV 89106, USA.
Biomedicines. 2023 Nov 9;11(11):3003. doi: 10.3390/biomedicines11113003.
This study sought to evaluate the expression of previously identified microRNAs known to regulate neuronal differentiation in mesenchymal stem cells (MSCs), including miR-27, miR-125, miR-128, miR-135, miR-140, miR-145, miR-218 and miR-410, among dental pulp stem cells (DPSCs) under conditions demonstrated to induce neuronal differentiation. Using an approved protocol, = 12 DPSCs were identified from an existing biorepository and treated with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF), which were previously demonstrated to induce neural differentiation markers including Sox1, Pax6 and NFM among these DPSCs. This study revealed that some microRNAs involved in the neuronal differentiation of MSCs were also differentially expressed among the DPSCs, including miR-27 and miR-145. In addition, this study also revealed that administration of bFGF and EGF was sufficient to modulate miR-27 and miR-145 expression in all of the stimulus-responsive DPSCs but not among all of the non-responsive DPSCs-suggesting that further investigation of the downstream targets of these microRNAs may be needed to fully evaluate and understand these observations.
本研究旨在评估在已证实可诱导神经元分化的条件下，牙髓干细胞（DPSC）中先前已确定的、已知可调节间充质干细胞（MSC）神经元分化的微小RNA（miRNA）的表达情况，这些miRNA包括miR-27、miR-125、miR-128、miR-135、miR-140、miR-145、miR-218和miR-410。采用已获批的方案，从现有的生物样本库中鉴定出12个DPSC，并使用碱性成纤维细胞生长因子（bFGF）和表皮生长因子（EGF）进行处理，先前已证实这两种因子可在这些DPSC中诱导包括Sox1、Pax6和神经丝蛋白（NFM）在内的神经分化标志物。本研究表明，一些参与MSC神经元分化的miRNA在DPSC中也存在差异表达，包括miR-27和miR-145。此外，本研究还表明，给予bFGF和EGF足以调节所有刺激反应性DPSC中miR-27和miR-145的表达，但并非所有无反应性DPSC都如此——这表明可能需要进一步研究这些miRNA的下游靶点，以充分评估和理解这些观察结果。
