College of Life Science and Technology, Guangxi University, Nanning 530005, China.
J Vet Sci. 2022 Mar;23(2):e35. doi: 10.4142/jvs.21308.
The testis has been reported to be a naturally O-deprived organ, dimethyloxaloylglycine (DMOG) can inhibit hypoxia inducible factor-1alpha (HIF-1α) subject to degradation under normal oxygen condition in cells.
The objective of this study is to detect the effects of DMOG on the proliferation and differentiation of spermatogonial stem cells (SSCs) in Bama minipigs.
Gradient concentrations of DMOG were added into the culture medium, HIF-1α protein in SSCs was detected by western blot analysis, the relative transcription levels of the SSC-specific genes were analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Six days post-induction, the genes related to spermatogenesis were detected by qRT-PCR, and the DNA content was determined by flow cytometry.
Results revealed that the levels of HIF-1α protein increased in SSCs with the DMOG treatment in a dose-dependent manner. The relative transcription levels of SSC-specific genes were significantly upregulated ( < 0.05) by activating HIF-1α expression. The induction results showed that DMOG significantly increased ( < 0.05) the spermatogenesis capability of SSCs, and the populations of haploid cells significantly increased ( < 0.05) in DMOG-treated SSCs when compared to those in DMOG-untreated SSCs.
We demonstrate that DMOG can promote the spermatogenesis activity of SSCs.
睾丸被报道为一个自然缺氧的器官，二甲基草酰甘氨酸（DMOG）可抑制细胞在常氧条件下缺氧诱导因子-1α（HIF-1α）的降解。
本研究旨在检测 DMOG 对巴马小型猪精原干细胞（SSCs）增殖和分化的影响。
梯度浓度的 DMOG 被添加到培养基中，通过 Western blot 分析检测 SSCs 中的 HIF-1α 蛋白，使用定量逆转录聚合酶链反应（qRT-PCR）分析 SSC 特异性基因的相对转录水平。诱导 6 天后，通过 qRT-PCR 检测与精子发生相关的基因，并通过流式细胞术测定 DNA 含量。
结果表明，DMOG 处理可使 SSCs 中的 HIF-1α 蛋白水平呈剂量依赖性增加。激活 HIF-1α 表达可显著上调（<0.05）SSC 特异性基因的相对转录水平。诱导结果表明，与未处理的 DMOG 的 SSCs 相比，DMOG 显著增加（<0.05）了 SSCs 的精子发生能力，且 DMOG 处理的 SSCs 中的单倍体细胞群显著增加（<0.05）。
我们证明 DMOG 可以促进 SSCs 的精子发生活性。
