van Driel-Kulker A M, Eysackers M J, Dessing M T, Ploem J S
Cytometry. 1986 Nov;7(6):601-4. doi: 10.1002/cyto.990070616.
A simple method is described for the selection of tumor areas in paraffin blocks for cytometry. The surface of a paraffin block is stained with the fluorescent dye DAPI. By means of incident fluorescence microscopy on the cut surface of a total block, the tissue can be visualized. Location of the tumor area with the aid of conventional histopathological criteria is feasible using the adjacent section after hematoxylin-eosin staining as a diagnostic guideline. Once the position of the tumor area is determined, a small hollow bore, which is screwed in the objective holder, is pressed in the tissue. The depth is controlled by the microscrew of the microscope. After retraction of the bore, the block is removed, and a thick section is cut. The selected area can be processed for cytometry separately from the remaining tissue. The technique can be used either to enrich the sample to be analyzed with tumor cells or to analyze histopathologically different tumor compartments. Both flow and image cytometry can make use of this selection technique.
描述了一种用于在石蜡块中选择肿瘤区域进行细胞计数的简单方法。石蜡块表面用荧光染料4′,6-二脒基-2-苯基吲哚（DAPI）染色。通过对整个石蜡块切割面进行落射荧光显微镜观察，可以使组织可视化。借助苏木精-伊红染色后的相邻切片作为诊断指南，利用传统组织病理学标准确定肿瘤区域的位置是可行的。一旦确定了肿瘤区域的位置，将拧在物镜架上的一个小空心钻压入组织中。深度由显微镜的微调螺丝控制。钻取出后，将石蜡块取下，切出一个厚切片。所选区域可以与其余组织分开进行细胞计数处理。该技术既可以用于用肿瘤细胞富集待分析样本，也可以用于分析组织病理学上不同的肿瘤区域。流式细胞术和图像细胞术都可以利用这种选择技术。
