Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Mossoro, RN, Brazil.
Cryo Letters. 2023 Mar-Apr;44(2):110-108.
The synergistic action among the different extracellular cryoprotectants could improve somatic cell quality after thawing and provide bases for the formation of biobanks for red-rumped agoutis.
This study evaluated the interactions among sucrose (SUC) and concentrations of serum fetal bovine (FBS) on the cryopreservation of somatic cells derived from red-rumped agoutis.
Cells were cryopreserved with 10% dimethyl sulfoxide and different concentrations of FBS (10%, 40%, and 90%) with or without 0.2 M SUC, totaling six comparison groups. Non-cryopreserved cells were used as a control. Cells were evaluated for viability, metabolic activity, proliferative activity, reactive oxygen species (ROS), mitochondrial membrane potential and apoptosis levels.
No difference was observed among cryopreserved with DMSO containing (10FBS, 10FBS-SUC, 40FBS, 40FBS-SUC, 90FBS, 90FBS-SUC) and non-cryopreserved groups for viability, metabolic activity, proliferative activity, and ROS levels. Interestingly, only cells cryopreserved with 90% FBS and SUC maintained the mitochondrial membrane potential like the control. This indicates that at high concentrations of FBS, SUC contributes to the maintenance of this parameter in cryopreserved cells. Moreover, at concentrations of 10% and 40% of FBS, SUC contributed to the maintenance of viability evaluated by the levels of apoptosis evaluated after thawing. In summary, we verified that 90% FBS and 0.2 M SUC promote greater ability of cells after thawing. Additionally, SUC positively acts in cryopreservation solutions containing 10% and 40% FBS.
This information is essential to an understanding of the mechanisms involved in the interactions of extracellular cryoprotectants in somatic cell cryopreservation solutions of red-rumped agoutis. DOI: 10.54680/fr23210110212.
不同细胞外冷冻保护剂的协同作用可以提高解冻后体细胞的质量，为红腹卷尾猴生物库的形成提供基础。
本研究评估了蔗糖（SUC）与胎牛血清（FBS）浓度在红腹卷尾猴体细胞冷冻保存中的相互作用。
使用含有 10％二甲基亚砜和不同浓度 FBS（10％、40％和 90％）的冷冻保护剂对细胞进行冷冻保存，其中添加或不添加 0.2 M SUC，共设置 6 个比较组。未冷冻保存的细胞作为对照。评估细胞活力、代谢活性、增殖活性、活性氧（ROS）、线粒体膜电位和凋亡水平。
在含有 DMSO 的冷冻保护剂（10FBS、10FBS-SUC、40FBS、40FBS-SUC、90FBS、90FBS-SUC）和未冷冻保存组之间，细胞活力、代谢活性、增殖活性和 ROS 水平均无差异。有趣的是，只有用 90％FBS 和 SUC 冷冻保存的细胞保持与对照组相似的线粒体膜电位。这表明，在高浓度的 FBS 下，SUC 有助于维持冷冻保存细胞中该参数。此外，在 10％和 40％FBS 浓度下，SUC 有助于维持解冻后通过凋亡水平评估的细胞活力。总之，我们验证了 90％FBS 和 0.2 M SUC 可提高解冻后细胞的活力。此外，SUC 在含有 10％和 40％FBS 的冷冻保护剂溶液中发挥积极作用。
这些信息对于理解红腹卷尾猴体细胞冷冻保护剂在冷冻保护剂溶液中的相互作用机制至关重要。DOI：10.54680/fr23210110212。
