Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Oral and Maxillofacial Surgery Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Photochem Photobiol. 2022 Nov;98(6):1434-1440. doi: 10.1111/php.13627. Epub 2022 Apr 20.
Peripheral nerve damages are among the most important consequences of dental and maxillofacial procedures. Tissue engineering using mesenchymal stem cells (MSCs) is a promising method to manage such injuries. Moreover, photobiomodulation therapy (PBMT) can enhance this treatment. The present study aimed to investigate the effect of PBMT on differentiation of MSCs derived from dental follicle (DF) into neurons. MSCs were isolated from an impacted tooth follicle by digestion method. The stem cells were cultured, and differentiated into neurons. The cells received two sessions of PBMT with 810 or 980 nm diode laser (100 mW, 4 J cm ) in either DMEM or neural inductive medium. Phenotypic characterization of the cells was determined using flow cytometry. In addition, β-tubulin and MAP2 genes expression level changes were analyzed using RT-PCR and western blot technique. After 14 days, flow cytometry analysis confirmed the mesenchymal nature of cells. RT-PCR and western blot affirmed the expression of β-tubulin and MAP2 genes and proteins respectively. PBMT with both wavelengths significantly increased β-tubulin and MAP2 expression in neural inductive medium with highest expression mean in 980 nm group. PBMT with 810 and 980 nm lasers could be a promising adjunctive method in differentiation of DF-originated MSCs into neural cells.
周围神经损伤是口腔颌面手术的重要后果之一。使用间充质干细胞（MSCs）的组织工程是管理此类损伤的一种有前途的方法。此外，光生物调节疗法（PBMT）可以增强这种治疗效果。本研究旨在探讨 PBMT 对来源于牙囊（DF）的 MSCs 向神经元分化的影响。通过消化法从阻生牙的牙囊分离出 MSC。培养干细胞并分化为神经元。细胞在 DMEM 或神经诱导培养基中接受两次 810 或 980nm 二极管激光（100mW，4J/cm）的 PBMT。使用流式细胞术测定细胞的表型特征。此外，使用 RT-PCR 和 Western blot 技术分析 β-微管蛋白和 MAP2 基因表达水平的变化。14 天后，流式细胞术分析证实了细胞的间充质性质。RT-PCR 和 Western blot 分别证实了 β-微管蛋白和 MAP2 基因和蛋白的表达。两种波长的 PBMT 均能显著增加神经诱导培养基中 β-微管蛋白和 MAP2 的表达，其中 980nm 组的表达均值最高。810nm 和 980nm 激光的 PBMT 可能是将 DF 来源的 MSC 分化为神经细胞的一种有前途的辅助方法。
