Dzau V J, Gonzalez D, Ellison K, Churchill S, Emmett N
Endocrinology. 1987 Jan;120(1):358-64. doi: 10.1210/endo-120-1-358.
Using specific antibody raised against renal renin, we have documented that the majority of the uterine renin-like activity in gravid and nongravid uteri is immunoreactive renin. To characterize its physiochemical properties, we obtained highly purified uterine renin by two affinity chromatographic steps, pepstatin and antirenin. Uterine renin has a pH optimum of 6, an apparent mol wt of 38K, and a Km of 1.7 microM for homologous substrate. These properties are identical to those of renal renin and are not influenced by the pregnant state. In the basal state, an inactive form of the uterine enzyme constitute 55 +/- 10% of the total uterine renin. During pregnancy, active renin increased 40-fold as inactive renin fell to 4 +/- 3% of the total renin concentration. The renal renin concentration fell as plasma renin increased during pregnancy. These data suggest that the increased uterine renin concentrations during pregnancy are probably due to increased local production and conversion of renin precursor to the active enzyme. This stimulation of the uterine renin level appears to be independent of renal renin.
利用针对肾素产生的特异性抗体，我们已证明妊娠子宫和非妊娠子宫中大部分子宫肾素样活性是免疫反应性肾素。为了表征其理化性质，我们通过胃蛋白酶抑制剂和抗肾素两步亲和色谱法获得了高度纯化的子宫肾素。子宫肾素的最适pH为6，表观分子量为38K，对同源底物的Km为1.7微摩尔。这些性质与肾素相同，且不受妊娠状态影响。在基础状态下，子宫酶的无活性形式占子宫肾素总量的55±10%。妊娠期间，活性肾素增加40倍，而无活性肾素降至总肾素浓度的4±3%。妊娠期间，随着血浆肾素增加，肾肾素浓度下降。这些数据表明，妊娠期间子宫肾素浓度增加可能是由于肾素前体的局部产生增加以及向活性酶的转化增加。子宫肾素水平的这种刺激似乎与肾肾素无关。
