Vallarino M, Uva B, Ghiani P
Biochim Biophys Acta. 1982 Mar 18;702(1):1-5. doi: 10.1016/0167-4838(82)90020-6.
Renin from rabbit kidney and uterus was extracted and purified by combined gel filtration, affinity chromatography and preparative isoelectric focusing; specific activities were determined by radioimmunoassay methods. The characterization was carried out by molecular weight determinations, polyacrylamide gel electrophoresis and analytical isoelectric focusing. A single form of renin was obtained from kidney extracts (purification 87 000-fold). From uterine extracts, however, four fractions were separated (purification 94 000; 30 000; 47 000; 187 000-fold, respectively). These fractions possess the same molecular weight but differences in electrophoretic mobilities and in isoelectric points were observed. Only one uterine fraction was coincident with the renal fraction. The uterine enzyme possesses a higher specific activity. It must be regarded as a local product of the uterine wall with a molecular configuration different from that of the renal renin.
采用凝胶过滤、亲和层析和制备性等电聚焦相结合的方法，从兔肾和子宫中提取并纯化了肾素；通过放射免疫分析方法测定了其比活性。通过分子量测定、聚丙烯酰胺凝胶电泳和分析性等电聚焦对其进行了表征。从肾提取物中获得了单一形式的肾素（纯化87000倍）。然而，从子宫提取物中分离出了四个组分（纯化倍数分别为94000、30000、47000、187000倍）。这些组分具有相同的分子量，但在电泳迁移率和等电点方面存在差异。只有一个子宫组分与肾组分一致。子宫酶具有较高的比活性。它必须被视为子宫壁的局部产物，其分子构型与肾素不同。
