文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

CDC42EP3通过调控细胞周期蛋白D1促进神经胶质瘤进展。

CDC42EP3 promotes glioma progression via regulation of CCND1.

作者信息

Yang Zhigang, Xu Tao, Xie Tao, Yang Liangliang, Wang Guiping, Gao Yang, Xi Gangming, Zhang Xiaobiao

机构信息

Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Department of Neurosurgery, Changzheng Hospital, Naval Medical University, Shanghai, China.

出版信息

Cell Death Dis. 2022 Apr 1;13(4):290. doi: 10.1038/s41419-022-04733-9.

DOI:10.1038/s41419-022-04733-9
PMID:35365622
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8975815/
Abstract

Gliomas are the most common brain malignancies characterized by high degree of aggressiveness and high mortality. However, the underlying mechanism of glioma progression remains unclear. Here, we probed the role of CDC42EP3 (CDC42 effector protein 3) played in glioma development and its potential downstream mechanism. The expression of CDC42EP3 in tumor and normal brain tissues were examined through immunohistochemistry and we found the likelihood of CDC42EP3 overexpression was positively correlated with pathological grading. Patients with higher expression of CDC42EP3 were more likely to suffer from recurrence as well. Through constructing CDC42EP3-knockdown cell models, we discovered that silencing CDC42EP3 significantly restricted cell proliferation and migration but facilitated cell apoptosis in vitro. Inhibition on tumor growth mediated by CDC42EP3 depletion was further verified in vivo. Regarding downstream target of CDC42EP3, we found that it may positively regulate the expression of CCND1 through c-Myc-mediated transcription. Furthermore, our findings affirmed that effects of CDC42EP3 overexpression on cell proliferation, migration and apoptosis could be confined by depleting CCND1. In a word, this study reported the tumor-promoting role of CDC42EP3 in glioma progression which probably functioned through targeting CCND1.

摘要

神经胶质瘤是最常见的脑恶性肿瘤，具有高度侵袭性和高死亡率。然而，神经胶质瘤进展的潜在机制仍不清楚。在此，我们探究了CDC42EP3（CDC42效应蛋白3）在神经胶质瘤发展中所起的作用及其潜在的下游机制。通过免疫组织化学检测了CDC42EP3在肿瘤组织和正常脑组织中的表达，我们发现CDC42EP3过表达的可能性与病理分级呈正相关。CDC42EP3表达较高的患者也更易复发。通过构建CDC42EP3敲低细胞模型，我们发现在体外沉默CDC42EP3可显著抑制细胞增殖和迁移，但促进细胞凋亡。在体内进一步验证了CDC42EP3缺失介导的对肿瘤生长的抑制作用。关于CDC42EP3的下游靶点，我们发现它可能通过c-Myc介导的转录正向调节CCND1的表达。此外，我们的研究结果证实，通过消耗CCND1可限制CDC42EP3过表达对细胞增殖、迁移和凋亡的影响。总之，本研究报道了CDC42EP3在神经胶质瘤进展中的促肿瘤作用，其可能通过靶向CCND1发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/98b43d2f4ef1/41419_2022_4733_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/f34da8b8c5b7/41419_2022_4733_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/aa08d127705f/41419_2022_4733_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/7f5adccdb657/41419_2022_4733_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/39c9228d66eb/41419_2022_4733_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/c6368782e60a/41419_2022_4733_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/98b43d2f4ef1/41419_2022_4733_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/f34da8b8c5b7/41419_2022_4733_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/aa08d127705f/41419_2022_4733_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/7f5adccdb657/41419_2022_4733_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/39c9228d66eb/41419_2022_4733_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/c6368782e60a/41419_2022_4733_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af7c/8975815/98b43d2f4ef1/41419_2022_4733_Fig6_HTML.jpg

相似文献

[1]
CDC42EP3 promotes glioma progression via regulation of CCND1.

Cell Death Dis. 2022-4-1

[2]
Deduction of CDC42EP3 suppress development and progression of osteosarcoma.

Exp Cell Res. 2022-3-1

[3]
CDC42EP3 is a key promoter involved in the development and progression of gastric cancer.

Carcinogenesis. 2021-10-5

[4]
Inhibition of Cyclin D1 Expression in Human Glioblastoma Cells is Associated with Increased Temozolomide Chemosensitivity.

Cell Physiol Biochem. 2018

[5]
Inhibition of EGR1 inhibits glioma proliferation by targeting CCND1 promoter.

J Exp Clin Cancer Res. 2017-12-15

[6]
MicroRNA‑195 suppresses cell proliferation, migration and invasion in epithelial ovarian carcinoma via inhibition of the CDC42/CCND1 pathway.

Int J Mol Med. 2020-11

[7]
GATAD1 gene amplification promotes glioma malignancy by directly regulating CCND1 transcription.

Cancer Med. 2019-7-8

[8]
MicroRNA-519d-3p inhibits cell proliferation and cell cycle G1/S transition in glioma by targeting CCND1.

Biosci Biotechnol Biochem. 2020-2

[9]
CDCA7L promotes glioma proliferation by targeting CCND1 and predicts an unfavorable prognosis.

Mol Med Rep. 2019-6-5

[10]
MicroRNA-383 inhibits anchorage-independent growth and induces cell cycle arrest of glioma cells by targeting CCND1.

Biochem Biophys Res Commun. 2014-10-31

引用本文的文献

[1]
Integrative analysis reveals key molecular mechanisms and prognostic model for Ethylnitrosourea-induced gliomagenesis.

BMC Pharmacol Toxicol. 2025-4-22

[2]
A novel peptide RR-171 derived from human umbilical cord serum induces apoptosis and pyroptosis in pancreatic cancer cells.

Sci Rep. 2025-4-14

[3]
Single-cell Analysis Highlights Anti-apoptotic Subpopulation Promoting Malignant Progression and Predicting Prognosis in Bladder Cancer.

Cancer Inform. 2025-2-26

[4]
Cdc42EP3-bound septin scaffolds promote actin polymerization.

J Biol Chem. 2025-3

[5]
TP53 and EGFR amplification are negative predictors of overall survival in patients diagnosed with non-small cell lung cancer with brain metastases.

Heliyon. 2024-8-19

[6]
The Role of the PAX Genes in Renal Cell Carcinoma.

Int J Mol Sci. 2024-6-19

[7]
AURKB/CDC37 complex promotes clear cell renal cell carcinoma progression via phosphorylating MYC and constituting an AURKB/E2F1-positive feedforward loop.

Cell Death Dis. 2024-6-18

[8]
Cell division cycle 42 effector protein 4 inhibits prostate cancer progression by suppressing ERK signaling pathway.

Biomol Biomed. 2023-12-27

[9]
AKR1B10 inhibits the proliferation and metastasis of hepatocellular carcinoma cells by regulating the PI3K/AKT pathway.

Oncol Lett. 2023-11-15

[10]
IFN-γ Triggered IFITM2 Expression to Induce Malignant Phenotype in Elderly GBM.

J Mol Neurosci. 2023-12

本文引用的文献

[1]
A novel function for cyclin D1 as a transcriptional role in oncogenesis and tumor development by ChIP-Seq and RNA-Seq.

J Cancer. 2021-6-26

[2]
Molecular subversion of Cdc42 signalling in cancer.

Biochem Soc Trans. 2021-6-30

[3]
The 2021 WHO Classification of Tumors of the Central Nervous System: a summary.

Neuro Oncol. 2021-8-2

[4]
CDC42EP3 is a key promoter involved in the development and progression of gastric cancer.

Carcinogenesis. 2021-10-5

[5]
CDK8 maintains stemness and tumorigenicity of glioma stem cells by regulating the c-MYC pathway.

Oncogene. 2021-4

[6]
CDC42EP3 promotes colorectal cancer through regulating cell proliferation, cell apoptosis and cell migration.

Cancer Cell Int. 2021-3-16

[7]
Molecular Mechanisms of Treatment Resistance in Glioblastoma.

Int J Mol Sci. 2020-12-31

[8]
CBTRUS Statistical Report: Primary Brain and Other Central Nervous System Tumors Diagnosed in the United States in 2013-2017.

Neuro Oncol. 2020-10-30

[9]
A Cyclin D1-Dependent Transcriptional Program Predicts Clinical Outcome in Mantle Cell Lymphoma.

Clin Cancer Res. 2021-1-1

[10]
Transcription factor NFYA promotes G1/S cell cycle transition and cell proliferation by transactivating cyclin D1 and CDK4 in clear cell renal cell carcinoma.

Am J Cancer Res. 2020-8-1

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索