Ma Lin, Yang Fang, Liu Qing, Zhu Xu-Tao, Liu Xin, Jin Sen, Wang Hua-Dong, Pei Lei, Xu Fu-Qiang, Liu Hai-Xia
Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430033, China.
Shenzhen Key Laboratory of Viral Vectors for Biomedicine, the Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Curr Med Sci. 2022 Apr;42(2):417-425. doi: 10.1007/s11596-022-2571-y. Epub 2022 Apr 2.
Previous studies have shown that the autonomic nervous system (ANS), which can be affected by emotions, is important in the occurrence or progression of glaucoma. The autonomic innervation distributed in the anterior chamber (AC) structures might play an efferent role in the neural regulation of intraocular pressure (IOP). This study aimed to investigate the anatomic neural connection from the emotional brain to autonomic innervation in the AC.
A retrograde trans-multisynaptic pseudorabies virus encoded with an enhanced green fluorescent protein (PRV531) and non-trans-synaptic tracer FAST Dil were injected into the right eye of mice, respectively. Fluorescent localization in the emotional brain and preganglionic nuclei was studied. Five and a half days after PRV531 injection into the right AC, fluorescent signals were observed in several emotional brain regions, including the amygdala, agranular insular cortex, lateral septal nuclei, periaqueductal gray, and hypothalamus. Autonomic preganglionic nuclei, including Edinger-Westphal nucleus, superior salivatory nucleus, and intermediolateral nucleus, were labeled using PRV531.
The sensory trigeminal nuclei were not labeled using PRV531. The fluorescence signals in the nuclei mentioned above showed bilateral distribution, primarily on the ipsilateral side. Seven days after injecting FAST Dil into the AC, we observed no FAST Dil-labeled neurons in the central nervous system.
Our results indicate a neural connection from the emotional brain to autonomic innervation in the AC, which provides anatomical support for the emotional influence of IOP via the ANS.
既往研究表明，可受情绪影响的自主神经系统（ANS）在青光眼的发生或进展中起重要作用。分布于前房（AC）结构中的自主神经支配可能在眼压（IOP）的神经调节中发挥传出作用。本研究旨在探讨从情绪脑到AC自主神经支配的解剖学神经连接。
分别将编码增强型绿色荧光蛋白的逆行跨多突触伪狂犬病病毒（PRV531）和非跨突触示踪剂FAST Dil注入小鼠右眼。研究情绪脑和节前核中的荧光定位。将PRV531注入右侧AC五天半后，在包括杏仁核、无颗粒岛叶皮质、外侧隔核、导水管周围灰质和下丘脑在内的几个情绪脑区观察到荧光信号。使用PRV531标记自主神经节前核，包括动眼神经副核、上涎核和中间外侧核。
使用PRV531未标记感觉三叉神经核。上述核中的荧光信号呈双侧分布，主要在同侧。将FAST Dil注入AC七天后，我们在中枢神经系统中未观察到FAST Dil标记的神经元。
我们的结果表明从情绪脑到AC自主神经支配存在神经连接，这为通过ANS对IOP的情绪影响提供了解剖学支持。
