Kamaludin Zaleha, Siddig Alaa, Yaacob Najib Majdi, Lam Alfred K, Rahman Wan Faiziah Wan Abdul
Department of Pathology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan 16150, Malaysia.
Unit of Biostatistics and Research Methodology, Health Campus, Universiti Sains Malaysia, Kelantan 16150, Malaysia.
Pathophysiology. 2022 Jan 13;29(1):9-23. doi: 10.3390/pathophysiology29010002.
Biomarker identification is imperative for invasive breast carcinoma, which is more aggressive and associated with higher mortality and worse prognosis in younger patients (<45 years) than in older patients (>50 years). The current study aimed to investigate angiopoietin-like protein 4 (ANGPTL4) and insulin-like growth factor-1 (IGF-1) protein expression in breast tissue from young patients with breast carcinoma. Immunohistochemical staining was applied in formalin-fixed, paraffin-embedded samples of breast carcinoma tissue from young patients aged <45 years at the time of diagnosis. Both proteins were expressed in the majority of cases. The highest frequency of positive ANGPTL4 and IGF-1 expression was observed in the luminal A subtype, whereas the HER2-overexpression subtype exhibited the lowest expression frequency for both proteins. There was no significant association between ANGPTL4 (p = 0.897) and IGF-1 (p = 0.091) expression and molecular subtypes of breast carcinoma. The histological grade was a significant predictor of ANGPTL4 expression (grade 1 vs. grade 3, adjusted odds ratio = 12.39, p = 0.040). Therefore, ANGPTL-4 and IGF-1 expressions are common in young breast carcinoma tissue. There is a potential use of them as biomarkers in breast carcinoma.
生物标志物的鉴定对于浸润性乳腺癌至关重要，浸润性乳腺癌更具侵袭性，与年轻患者（<45岁）相比，老年患者（>50岁）的死亡率更高且预后更差。本研究旨在调查年轻乳腺癌患者乳腺组织中血管生成素样蛋白4（ANGPTL4）和胰岛素样生长因子-1（IGF-1）的蛋白表达情况。对诊断时年龄<45岁的年轻患者的福尔马林固定、石蜡包埋的乳腺癌组织样本进行免疫组织化学染色。在大多数病例中均检测到这两种蛋白的表达。ANGPTL4和IGF-1阳性表达频率最高的是管腔A型，而HER2过表达型这两种蛋白的表达频率最低。ANGPTL4（p = 0.897）和IGF-1（p = 0.091）的表达与乳腺癌分子亚型之间无显著关联。组织学分级是ANGPTL4表达的显著预测指标（1级与3级，校正比值比= 12.39，p = 0.040）。因此，ANGPTL-4和IGF-1在年轻乳腺癌组织中表达常见。它们有可能作为乳腺癌的生物标志物。
