添加铜和锌的卵泡液可提高小鼠玻璃化冷冻-解冻卵母细胞的成熟率。
Follicular fluid supplemented with copper and zinc increase the maturation rate of mouse vitrified-warmed oocytes.
机构信息
Department of Biology, Faculty of Basic Sciences, Razi University, Taq-e Bostan, Bagh-e Abrisham, 6714967346, Kermanshah, Iran.
Department of Anatomical Sciences and Biology, Faculty of Medicine, Kermanshah University of Medical Sciences, Shahid Beheshti Boulevard, 6715847141, Kermanshah, Iran.
出版信息
Cryo Letters. 2021 Nov-Dec;42(6):326-331.
BACKGROUND
Immature oocyte cryopreservation is a therapeutic option in assisted reproductive technology.
OBJECTIVE
To evaluate the effects of supplementing oocyte maturation medium with human follicular fluid (hFF), zinc and copper.
MATERIALS AND METHODS
Four different maturation media supplemented with 10% follicular fluid, 4 μg/mL copper and 1 μg/mL zinc were used for vitrified-warmed oocytes of mouse.
RESULTS
Maturation rate was the highest (63.3%) in the presence of zinc. Cleavage and blastocyst rates in groups with copper and zinc were significantly higher than the control group (39.9% and 46.4% vs. 28.8%), without any significant difference between Zn and Cu.
CONCLUSION
Our findings indicate the high importance of using hFF as a natural medium, and also zinc and copper as two efficient trace elements in the maturation medium for vitrified-warmed oocytes.
背景
未成熟卵母细胞冷冻保存是辅助生殖技术中的一种治疗选择。
目的
评估在卵母细胞成熟培养基中添加人卵泡液（hFF）、锌和铜对其的影响。
材料与方法
使用四种不同的成熟培养基，分别添加 10%卵泡液、4μg/mL 铜和 1μg/mL 锌，用于玻璃化冷冻-解冻的小鼠卵母细胞。
结果
锌存在时成熟率最高（63.3%）。添加铜和锌的组的卵裂和囊胚形成率明显高于对照组（39.9%和 46.4%比 28.8%），但锌和铜之间没有显著差异。
结论
我们的研究结果表明，使用 hFF 作为天然培养基，以及锌和铜作为成熟培养基中的两种有效微量元素，对玻璃化冷冻-解冻的卵母细胞非常重要。
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