Geravandi S, Kalehoei E, Karami A, Nowrouzi F, Kalhori Z, Zhaleh H, Azadbakht M
Department of Biology, Faculty of Basic Sciences, Razi University, Kermanshah, Iran.
Department of Anatomical Sciences and Biology, Faculty of Medicine, University of Medical Sciences, Kermanshah, Iran.
Cryo Letters. 2023 Mar-Apr;44(2):80-88.
In vitro maturation (IVM) and oocyte cryopreservation are therapeutic options in assisted reproductive technology which is used to preserve fertility in patients with different causes of infertility.
To analyze in vitro development of vitrified-warmed oocytes in the presence of human follicular fluid (FF) and bone marrow mesenchymal stem cell-conditioned medium (BMSC-CM) as a rescue strategy in fertility preservation.
BMSC-CM and FF media were used as two natural media. Not only osteogenic and adipogenic differentiation but also flow cytometry was carried out to confirm the nature of mesenchymal stem cells. A total of 327 vitrified-warmed oocytes were randomly assigned to three groups with different maturation media. After 24 h the maturation rate was evaluated. In vitro fertilization and also embryo development were also assessed.
Oocytes matured in the BMSC-CM and FF groups showed a significant increase compared to the control group (76.6+/-2.9, 53.2±1.0 , and 40.8+/-6.1, respectively) (P % 0.05). Embryo cleavage rates in the BMSC-CM were dramatically higher than FF and control groups (85.6+/-2.2, 70.5+/-2.2, and 60.7+/-1.5, respectively). Blastocyst formation rates in the BMSC-CM group were statically different compared to FF and control groups (73.6+/-1.0, 58.5+/-1.0, and 45.8+/-4.2, respectively).
BMSC-CM and FF media not only improve the maturation rate of vitrified warmed oocytes but also significantly increase embryo cleavage and blastocyst rates. DOI: 10.54680/fr23210110512.
体外成熟（IVM）和卵母细胞冷冻保存是辅助生殖技术中的治疗选择，用于为不同原因导致不孕的患者保留生育能力。
分析在人卵泡液（FF）和骨髓间充质干细胞条件培养基（BMSC-CM）存在的情况下，玻璃化冷冻-解冻卵母细胞的体外发育情况，作为生育力保存的一种挽救策略。
将BMSC-CM和FF培养基用作两种天然培养基。不仅进行了成骨和成脂分化，还通过流式细胞术来确认间充质干细胞的性质。总共327个玻璃化冷冻-解冻卵母细胞被随机分配到三组，分别使用不同的成熟培养基。24小时后评估成熟率。同时也评估了体外受精和胚胎发育情况。
与对照组相比，在BMSC-CM组和FF组中成熟的卵母细胞显著增加（分别为76.6±2.9、53.2±1.0和40.8±6.1）（P＜0.05）。BMSC-CM组的胚胎分裂率显著高于FF组和对照组（分别为85.6±2.2、70.5±2.2和60.7±1.5）。与FF组和对照组相比，BMSC-CM组的囊胚形成率有统计学差异（分别为73.6±1.0、58.5±1.0和45.8±4.2）。
BMSC-CM和FF培养基不仅提高了玻璃化冷冻-解冻卵母细胞的成熟率，还显著提高了胚胎分裂率和囊胚率。DOI: 10.54680/fr23210110512。
