Zhan Xin, Yu Xiaoxiao, Li Benmengyang, Zhou Rui, Fang Qingyu, Wu Yiwei
Hubei Collaborative Innovation Center for Rare Metal Chemistry, Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi 435002, China.
Hubei Collaborative Innovation Center for Rare Metal Chemistry, Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi 435002, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jul 5;275:121191. doi: 10.1016/j.saa.2022.121191. Epub 2022 Mar 25.
In the presence of thioglycolic acid (TGA) and under the catalysis of Fe, a simple, rapid, sensitive, selective and effective ratiometric fluorescence sensor platform based on the mixed physically blue nitrogen-doped graphene quantum dots (N-GQDs) as probe signals and orange rhodamine B as internal standard signals has been constructed for analysis of HO in human serum. TGA is the key factor for fluorescence response toward HO by N-GQDs and the mechanism is HO reacts speedily with TGA under the catalysis of Fe, and produces intermediate of superoxide anions (O), which accepts electrons from N-GQDs, and generates graphene oxide, causing the fluorescence quench of N-GQDs. Compared with N-GQDs probe, the sensitivity of the ratiometric fluorescence sensor platform of N-GQDs/rhodamine B for analysis of HO has been improved by nearly 5-folds. Under the optimum conditions, F/F has a good linear relationship with the concentration of HO and the detection limit of HO is 0.46 μmol/L with 3.5% RSD. The established sensor platform has been successfully used for probing HO in human serum with satisfactory results. The superior performance of the probe lies in its high selectivity and can be directly employed in detecting HO in serum samples without any sample pretreatment procedures.
在巯基乙酸（TGA）存在下，以铁为催化剂，构建了一种基于混合物理蓝色氮掺杂石墨烯量子点（N-GQDs）作为探针信号和橙色罗丹明B作为内标信号的简单、快速、灵敏、选择性好且有效的比率荧光传感器平台，用于分析人血清中的HO。TGA是N-GQDs对HO产生荧光响应的关键因素，其机制为HO在铁的催化下与TGA迅速反应，生成超氧阴离子（O）中间体，该中间体从N-GQDs接受电子，生成氧化石墨烯，导致N-GQDs荧光猝灭。与N-GQDs探针相比，N-GQDs/罗丹明B比率荧光传感器平台对HO分析的灵敏度提高了近5倍。在最佳条件下，F/F与HO浓度具有良好的线性关系，HO的检测限为0.46 μmol/L，相对标准偏差为3.5%。所建立的传感器平台已成功用于探测人血清中的HO，结果令人满意。该探针的卓越性能在于其高选择性，无需任何样品预处理程序即可直接用于检测血清样品中的HO。
