Zhang Fan, Jia Yong, Chen Fangman, Zhao Yawei, Li Li, Chang Zhimin
CAS Key Laboratory of Bio Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences, Suzhou, China.
Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China.
Front Bioeng Biotechnol. 2023 Aug 11;11:1226680. doi: 10.3389/fbioe.2023.1226680. eCollection 2023.
Fluorescent visualization of hydrogen peroxide in the tumor microenvironment (TME) is conducive to predicting malignant prognosis after chemotherapy. Two photon microscopy has been employed for hydrogen peroxide detection owing to its advantages of deep penetration and low phototoxicity. In this study, a two-photon fluorescent probe (TPFP) was protected by mesoporous silica nanoparticles (MSNs) and masked by cloaking the cancer cell membranes (CM), forming a tumor-targeted bioactive nanoprobe, termed MSN@TPFP@CM. This multifunctional nanoprobe allowed for the effective and selective detection of excessive hydrogen peroxide production in chemotherapeutic Etoposide (VP-16)-challenged tumor cells using two-photon microscopy. After specific accumulation in tumors, VP-16-MSN@TPFP@CM monitored tumor-specific hydrogen peroxide levels and revealed a positive correlation between oxidative stress in the TME and chemotherapy-exacerbated malignant prognosis. Given the recent translation of fluorescent imaging into early clinical trials and the high biocompatibility of bioactive nanoprobes, our approach may pave the way for specific imaging of oxidative stress in solid tumors after treatment and provide a promising technology for malignant prognosis predictions.
肿瘤微环境（TME）中过氧化氢的荧光可视化有助于预测化疗后的恶性预后。由于双光子显微镜具有深层穿透和低光毒性的优点，已被用于过氧化氢检测。在本研究中，一种双光子荧光探针（TPFP）被介孔二氧化硅纳米颗粒（MSNs）保护，并通过包裹癌细胞膜（CM）进行掩蔽，形成一种肿瘤靶向生物活性纳米探针，称为MSN@TPFP@CM。这种多功能纳米探针能够使用双光子显微镜有效且选择性地检测经化疗药物依托泊苷（VP-16）处理的肿瘤细胞中过量产生的过氧化氢。在肿瘤中特异性积累后，VP-16-MSN@TPFP@CM监测肿瘤特异性过氧化氢水平，并揭示TME中的氧化应激与化疗加剧的恶性预后之间存在正相关。鉴于荧光成像最近已转化为早期临床试验，且生物活性纳米探针具有高生物相容性，我们的方法可能为治疗后实体瘤中氧化应激的特异性成像铺平道路，并为恶性预后预测提供一项有前景的技术。
