嗜铁氧化硫杆菌结构固氮基因在大肠杆菌中的鉴定与克隆。
Identification and cloning of Thiobacillus ferrooxidans structural nif genes in Escherichia coli.
作者信息
Pretorius I M, Rawlings D E, Woods D R
出版信息
Gene. 1986;45(1):59-65. doi: 10.1016/0378-1119(86)90132-0.
The presence of nucleotide sequences which are homologous to the nifHDK genes of Klebsiella pneumoniae was demonstrated in total DNA preparations from five different iron-oxidizing Thiobacillus ferrooxidans strains. A non-iron-oxidizing Thiobacillus novellus strain and a heterotrophic Acidiphilium strain, which occurs in close association with T. ferrooxidans, did not contain DNA homologous to the nifHDK genes. The T. ferrooxidans ATCC33020 nifHDK genes were cloned and their arrangement was characterized on a 6.7-kb insert in pIMP16.
在从五种不同的氧化亚铁硫杆菌菌株制备的总DNA中，证实了存在与肺炎克雷伯氏菌nifHDK基因同源的核苷酸序列。一种非氧化亚铁的诺氏硫杆菌菌株和一种与氧化亚铁硫杆菌紧密相关的嗜酸性异养菌株，不含与nifHDK基因同源的DNA。克隆了氧化亚铁硫杆菌ATCC33020的nifHDK基因，并在pIMP16的一个6.7kb插入片段上对其排列进行了表征。
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