CLECEM group, Department of Analytical Chemistry, University of Valencia, University of Valencia, C/Doctor Moliner 50, 46100, Burjassot Valencia, Spain.
Department of Chemistry, Technical University of Munich, Elisabeth-Winterhalter Str. 6, 83177, München, Germany.
Mikrochim Acta. 2022 Apr 2;189(5):173. doi: 10.1007/s00604-022-05267-9.
This article reports current research efforts towards designing bespoke microscale extraction approaches exploiting the versatility of 3D printing for fast prototyping of novel geometries of sorptive devices. This is demonstrated via the so-called 3D printed spinning cup-based platform for immunoextraction of emerging contaminants using diclofenac as a model analyte. A new format of rotating cylindrical scaffold (containing a semispherical upper cavity) with enhanced coverage of biorecognition elements, and providing elevated enhancement factors with no need of eluate processing as compared with other microextraction stirring units is proposed. Two distinct synthetic routes capitalized upon modification of the acrylate surface of stereolithographic 3D printed parts with hexamethylenediamine or branched polyethyleneimine chemistries were assayed for covalent binding of monoclonal diclofenac antibody.Under the optimized experimental conditions, a LOD of 108 ng L diclofenac, dynamic linear range of 0.4-1,500 µg L, and enrichment factors > 83 (for near-exhaustive extraction) were obtained using liquid chromatography coupled with UV-Vis detection. The feasibility of the antibody-laden device for handling of complex samples was demonstrated with the analysis of raw influent wastewaters with relative recoveries ranging from 102 to 109%. By exploiting stereolithographic 3D printing, up to 36 midget devices were fabricated in a single run with an estimated cost of mere 0.68 euros per 3D print and up to 16 €/device after the incorporation of the monoclonal antibody.
本文报道了当前设计定制微尺度提取方法的研究工作，这些方法利用 3D 打印的多功能性快速原型制作新型吸附装置几何形状。这是通过所谓的 3D 打印旋转杯基平台实现的，该平台用于使用双氯芬酸作为模型分析物免疫提取新兴污染物。提出了一种新的旋转圆柱形支架格式（包含半圆形上腔），其生物识别元素的覆盖范围得到增强，并提供了更高的增强因子，与其他微萃取搅拌单元相比，无需进行洗脱液处理。利用立体光刻 3D 打印零件的丙烯酸盐表面与己二胺或支化聚乙烯亚胺化学物质进行修饰，测试了两种不同的合成途径，用于共价键合单克隆双氯芬酸抗体。在优化的实验条件下，使用液相色谱-紫外可见检测法，可获得 108ng L 的双氯芬酸的检测限、0.4-1500μg L 的动态线性范围和>83 的富集因子（用于近乎完全提取）。通过使用负载抗体的装置处理复杂样品的可行性进行了演示，分析了原始废水进水的相对回收率在 102%至 109%之间。通过利用立体光刻 3D 打印，可以在单个运行中制造多达 36 个微型装置，每个 3D 打印的估计成本仅为 0.68 欧元，而在加入单克隆抗体后，每个装置的成本高达 16 欧元。
