State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China.
Section of Transcription & Gene Regulation, The Hormel Institute, University of Minnesota, Austin, MN 55912, USA.
Trends Biochem Sci. 2022 Aug;47(8):710-724. doi: 10.1016/j.tibs.2022.03.007. Epub 2022 Mar 31.
Transcription is an essential and multistep process carried out by RNA polymerase (RNAP). In bacterial RNAP, in addition to the catalytic core domain, multiple other conserved domains are also identified to play regulatory roles in transcription. One such domain is the zinc-binding domain (ZBD) located at the N terminus of the largest subunit β' in bacterial RNAP, whose homolog is also reported in eukaryotic RNAPs. Recent structural and biochemical studies have revealed various key roles of the conserved β' ZBD during different steps of transcription. In this review, we summarize recent progress on the regulatory roles of this β' ZBD in bacterial transcription.
转录是由 RNA 聚合酶 (RNAP) 进行的一个基本且多步骤的过程。在细菌 RNAP 中，除了催化核心结构域外，还鉴定出多个其他保守结构域在转录中发挥调节作用。其中一个结构域是锌结合结构域 (ZBD)，位于细菌 RNAP 大亚基β'的 N 端，真核 RNAP 中也有其同源物。最近的结构和生化研究揭示了该保守β'ZBD 在转录的不同步骤中的各种关键作用。在这篇综述中，我们总结了该β'ZBD 在细菌转录中的调节作用的最新进展。
