Le Xuan Chinh, Wannavijit Supreya, Outama Piyatida, Montha Napatsorn, Lumsangkul Chompunut, Tongsiri Sudaporn, Chitmanat Chanagun, Hoseinifar Seyed Hossein, Van Doan Hien
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand.
Faculty of Fisheries Technology and Aquatic Resources, Maejo University, Chiang Mai, 50290, Thailand.
Fish Shellfish Immunol. 2022 May;124:134-141. doi: 10.1016/j.fsi.2022.03.039. Epub 2022 Mar 31.
This study aimed to evaluate the effects of rambutan peel powder (RP) on growth, skin mucosal and serum immunities, and immune-related gene expression of striped catfish (Pangasianodon hypophthalmus) reared in a biofloc system. Three hundred fingerlings (17.14 ± 0.12 g fish) were randomly selected and assigned to five treatments corresponding to five diets: 0 g kg (control - RP0); 10 g kg (RP10); 20 g kg (RP20); 40 g kg (RP40), and 80 g kg (RP80) for 8 weeks. At weeks 4 and 8 post-feeding, growth, skin mucus, and serum immunity parameters were determined, whereas immune-related gene expressions were performed at the end of the feeding trial. Based on the results, skin mucus lysozyme (SML) and skin mucus peroxidase (SMP) were significantly higher in fish fed the RP diets compared to the control diet (P < 0.05). The highest SML and SMP levels were observed in fish fed RP40 diet, followed by RP20, RP80, RP10, and RP0. Fish-fed RP diets had higher serum lysozyme and serum peroxidase activities, with the highest value found in the RP40 diet (P < 0.05), followed by RP20, RP80, and RP10. Similarly, immune-related gene expressions (IFN2a, IFN2b, and MHCII) in the liver were significantly up-regulated in fish fed RP40. Up-regulation (P < 0.05) of IL-1, IFN2a, IFN2b, and MHCII genes was also observed in fish intestines, with the highest values observed in fish fed RP40 diet, followed by RP10, RP20, RP80, and RP0. Fish-fed diet RP diets also showed enhanced growth and FCR compared to the control, with the highest values observed in fish fed diet RP40. However, no significant differences in survival rates were found among diets. In conclusion, dietary inclusion of RP at 40 g kg resulted in better growth performance, immune response, and immune related gene expressions of striped catfish (Pangasianodon hypophthalmus).
本研究旨在评估红毛丹果皮粉（RP）对生物絮团系统中养殖的苏氏圆腹芒（Pangasianodon hypophthalmus）生长、皮肤黏膜和血清免疫力以及免疫相关基因表达的影响。随机选取300尾鱼种（平均体重17.14 ± 0.12克），分为五组，分别投喂五种日粮：0克/千克（对照组 - RP0）；10克/千克（RP10）；20克/千克（RP20）；40克/千克（RP40）和80克/千克（RP80），为期8周。在投喂后第4周和第8周，测定生长、皮肤黏液和血清免疫参数，而免疫相关基因表达在投喂试验结束时进行。结果显示，与对照组日粮相比，投喂RP日粮的鱼皮肤黏液溶菌酶（SML）和皮肤黏液过氧化物酶（SMP）显著更高（P < 0.05）。在投喂RP40日粮的鱼中观察到最高的SML和SMP水平，其次是RP20、RP80、RP10和RP0。投喂RP日粮的鱼血清溶菌酶和血清过氧化物酶活性更高，RP40日粮组的值最高（P < 0.05），其次是RP20、RP80和RP10。同样，在投喂RP40的鱼肝脏中，免疫相关基因表达（IFN2a、IFN2b和MHCII）显著上调。在鱼肠道中也观察到IL-1、IFN2a、IFN2b和MHCII基因上调（P < 0.05），投喂RP40日粮的鱼中这些基因的值最高，其次是RP10、RP20、RP80和RP0。与对照组相比，投喂RP日粮的鱼生长和饲料系数也有所提高，投喂RP40日粮的鱼中这些值最高。然而，各日粮组之间的存活率没有显著差异。总之，日粮中添加40克/千克的RP可使苏氏圆腹芒（Pangasianodon hypophthalmus）具有更好的生长性能、免疫反应和免疫相关基因表达。
