East China Sea Fisheries Research Institute, China Academy of Fishery Sciences, Shanghai 200090, China.
East China Sea Fisheries Research Institute, China Academy of Fishery Sciences, Shanghai 200090, China.
J Invertebr Pathol. 2022 May;190:107752. doi: 10.1016/j.jip.2022.107752. Epub 2022 Apr 1.
Macrobrachium rosenbergii is an important economic aquatic animal and has been cultivated worldwide. However, it has suffered a disease of precocious puberty and growth retardation. This disease was also called iron prawn syndrome (IPS) in recent years. However, the cause of this disease has thus far remained a mystery . The present work utilized transcriptome sequencing technology to acquire gene expression information of gonads and to find the differentially expressed genes(DEGs) between diseased and normal male prawn. Finally, 426 significantly expressed genes were identified(p less than 0.01, |log2FC|≥1), of which 171 genes were up-regulated and 255 were down-regulated. Furthermore, DEGs were annotated to 36 GO terms and 202 KEGG pathways. Enrichment analysis of DEGs resulted in 10 significantly enriched GO terms and a total of 12 significantly enriched KEGG pathways. Analysis of the transcriptome sequences and DEGs identified several unigenes and pathways involved in precocious puberty and growth retardation. Quantitative PCR was performed to validate accuracy of the RNA-seq and the expression level of 10 genes, calculated by two analysis method, was mostly consistent. This is the first time to report precocious puberty and growth retardation male M. rosenbergii by transcriptome sequencing. The data presented here reveals key insights into the genetic markers of precocious puberty and growth retardation of male M. rosenbergii.
罗氏沼虾是一种重要的经济水生动物，已在全球范围内进行养殖。然而，它已经遭受了早熟和生长迟缓的疾病。近年来，这种疾病也被称为铁虾综合征（IPS）。然而，迄今为止，这种疾病的病因仍然是个谜。本研究利用转录组测序技术获取性腺的基因表达信息，并找到患病和正常雄性对虾之间的差异表达基因（DEGs）。最后，鉴定出 426 个显著表达基因（p 值小于 0.01，|log2FC|≥1），其中 171 个基因上调，255 个基因下调。此外，DEGs 被注释到 36 个 GO 术语和 202 个 KEGG 途径。DEGs 的富集分析得到了 10 个显著富集的 GO 术语和总共 12 个显著富集的 KEGG 途径。对转录组序列和 DEGs 的分析鉴定了一些与早熟和生长迟缓相关的 unigenes 和途径。通过 qPCR 验证了 RNA-seq 的准确性，两种分析方法计算的 10 个基因的表达水平基本一致。这是首次通过转录组测序报告雄性罗氏沼虾的早熟和生长迟缓。本研究结果揭示了雄性罗氏沼虾早熟和生长迟缓的遗传标记的关键信息。
