College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; Key Laboratory of Water and Sediment Sciences, Ministry of Education of China, Beijing 100871, China.
College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China; State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems, Beijing 100871, China.
Bioresour Technol. 2022 May;352:127099. doi: 10.1016/j.biortech.2022.127099. Epub 2022 Mar 31.
Although amino acid (AA) metabolism is basis of bacterial activities, unique characteristics of its response to decreased temperatures are not fully understood. Achieving nitrogen removal rate of 130-150 mg N/ (L∙d), metabolic differences of anammox consortia between 35 °C and four decreased temperatures (15-30 °C) were revealed respectively. 0-11.4-fold abundance variation of marker metabolites evidenced change of key metabolism (metabolism of AA, lipid and energy production) at decreased temperatures. However, AA metabolism varied more obviously than others, implying stronger response and higher functional potential. Efficiently, network topology confirmed more cellular processes represented by growth metabolism and biofilm formation were influenced by AA metabolism. Flexibly, down-regulated biosynthesis of unfavorable AAs for psychrophilic enzyme differed from enhanced biosynthesis of costly AAs, which only matched partial decreased temperatures to save energy. This work elucidates advantages of AA metabolism over others, exogenous amino acids could significantly promote activity of anammox bacteria at decreased temperatures.
尽管氨基酸（AA）代谢是细菌活动的基础，但人们对其对低温响应的独特特征还不完全了解。实现了 130-150 mg N/（L·d）的氮去除率，分别揭示了 anammox 菌落在 35°C 和四个低温（15-30°C）下的代谢差异。标记代谢物的丰度变化 0-11.4 倍表明关键代谢（AA、脂质和能量产生的代谢）在低温下发生变化。然而，AA 代谢的变化比其他代谢更为明显，这意味着更强的反应和更高的功能潜力。有效，网络拓扑结构证实更多受生长代谢和生物膜形成代表的细胞过程受到 AA 代谢的影响。灵活地，对低温酶不利的 AA 的生物合成下调与昂贵 AA 的增强生物合成不同，这仅与部分低温相匹配以节省能量。这项工作阐明了 AA 代谢相对于其他代谢的优势，外源性氨基酸可以显著促进 anammox 菌在低温下的活性。
