School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, PR China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, PR China; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, Hunan 410125, PR China.
School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, PR China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, PR China; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha, Hunan 410125, PR China.
Water Res. 2022 Oct 1;224:119062. doi: 10.1016/j.watres.2022.119062. Epub 2022 Sep 6.
Denitrifying bacteria with high abundances in anammox communities play crucial roles in achieving stable anammox-based systems. Despite the relative constant composition of denitrifying bacteria, their functional diversity remains to be explored in anammox communities. Herein, a total of 77 high-quality metagenome-assembled genomes (MAGs) of denitrifying bacteria were recovered from the anammox community in a full-scale swine wastewater treatment plant. Among these microbes, a total of 26 MAGs were affiliated with the seven dominant denitrifying genera that have total abundances higher than 1%. A meta-analysis of these species suggested that external organics reduced the abundances of genus Ignavibacterium and species MAG.305 of UTPRO2 in anammox communities. Comparative genome analysis revealed functional divergence across different denitrifying bacteria, largely owing to their distinct capabilities for carbohydrate (including endogenous and exogenous) utilization and vitamin (e.g., pantothenate and thiamine) biosynthesis. Serval microbes in this system contained fewer genes encoding biotin, pantothenate and methionine biosynthesis compared with their related species from other habitats. In addition, the genes encoding energy production and conversion (73 genes) and inorganic ion transport (53 genes) putatively transferred from other species to denitrifying bacteria, while these denitrifying bacteria (especially genera UTPRO2 and SCN-69-89) likely donated the genes encoding nutrients (e.g., inorganic ion and amino acid) transporter (64 genes) for other members to utilize new metabolites. Collectively, these findings highlighted the functional divergence of these denitrifying bacteria and speculated that the genetic interactions within anammox communities through horizontal gene transfer may be one of the reasons for their functional divergence.
在厌氧氨氧化系统中丰度较高的反硝化细菌在实现稳定的厌氧氨氧化系统中发挥着关键作用。尽管反硝化细菌的相对组成保持不变，但它们在厌氧氨氧化群落中的功能多样性仍有待探索。在此，从一个全规模的猪场废水处理厂的厌氧氨氧化群落中回收了总共 77 个高质量的反硝化菌宏基因组组装基因组（MAG）。在这些微生物中，共有 26 个 MAG 与 7 个丰度高于 1%的主要反硝化属有关。对这些物种的元分析表明，外部有机物降低了属 Ignavibacterium 和 UTPRO2 种 MAG.305 的丰度。比较基因组分析揭示了不同反硝化细菌之间的功能分化，这主要归因于它们在碳水化合物（包括内源性和外源性）利用和维生素（如泛酸和硫胺素）生物合成方面的不同能力。该系统中的一些微生物与其他生境中的相关物种相比，编码生物素、泛酸和蛋氨酸生物合成的基因较少。此外，编码能量产生和转化（73 个基因）和无机离子运输（53 个基因）的基因可能从其他物种转移到反硝化细菌，而这些反硝化细菌（特别是 UTPRO2 和 SCN-69-89 属）可能捐赠了编码营养物质（如无机离子和氨基酸）转运体（64 个基因）的基因，以供其他成员利用新的代谢物。总的来说，这些发现强调了这些反硝化细菌的功能分化，并推测通过水平基因转移在厌氧氨氧化群落中发生的遗传相互作用可能是它们功能分化的原因之一。
